Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 238-830-2
CAS number: 14765-30-1
The mean tissue viability obtained after 15 ± 0.5 minutes treatment with
FRESKOMENTHE was compared to the negative control tissues. Skin
irritation is expressed as the remaining cell viability after exposure
to the test item. The relative mean tissue viability obtained after 15 ±
0.5 minutes treatment with FRESKOMENTHE compared to the negative control
tissues was 8%. Since the mean relative tissue viability for
FRESKOMENTHE was below 50% it is considered to be irritant.
In vitro skin irritation test with FRESKOMENTHE using a human skin model.
This report describes the ability of FRESKOMENTHE to induce skin
irritation on a human three dimensional epidermal model (EPISKIN Small
Model (EPISKIN-SMTM)). The possible skin irritation potential of
FRESKOMENTHE was tested through topical application for 15 minutes.
The study procedures described in this report were based on the most
recent OECD and EC guidelines.
Batch VE00411894 of FRESKOMENTHE was a clear colourless liquid.
FRESKOMENTHE was applied undiluted (25 μl), directly on top of the skin
tissue for 15 ± 0.5 minutes.
After a 42 hour post-incubation period, determination of the cytotoxic
(irritancy) effect was performed. Cytotoxicity is expressed as the
reduction of mitochondrial dehydrogenase activity measured by formazan
production from MTT at the end of the treatment.
Skin irritation is expressed as the remaining cell viability after
exposure to the test item. The relative mean tissue viability obtained
after 15 ± 0.5 minutes treatment with FRESKOMENTHE compared to the
negative control tissues was 8%. Since the mean relative tissue
viability for FRESKOMENTHE was below 50% after 15 ± 0.5 minutes
treatment it is considered to be irritant.
The positive control had a mean cell viability of 27% after 15 ± 0.5
minutes exposure. The absolute mean OD570 (optical density at 570 nm) of
the negative control tissues was within the laboratory historical
control data range. The standard deviation value of the percentage
viability of three tissues treated identically was less than 19%,
indicating that the test system functioned properly (study plan
Finally, it is concluded that this test is valid and that FRESKOMENTHE
is irritant in the in vitro skin irritation test under the experimental
conditions described in this report
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again