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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from Secondary literature

Data source

Reference
Reference Type:
secondary source
Title:
Gene mutation in vitro toxicity assay for Ammonium molybdate- Molybdenum and molybdenum compounds
Author:
Gene mutation in vitro toxicity assay for Ammonium molybdate- Molybdenum and molybdenum compounds
Year:
2013
Bibliographic source:
Health Council of Netherlands, U-7988/JR/fs/459-B69, 2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Refer below principle
Principles of method if other than guideline:
In vitro mammalian chromosome aberration test was performed to evaluate the mutagenic nature of ammonium molybdate
GLP compliance:
not specified
Type of assay:
other: Chromosomal aberration assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium molybdate(VI)
EC Number:
236-031-3
EC Name:
Ammonium molybdate(VI)
Cas Number:
13106-76-8
Molecular formula:
H4N.1/2MoO4
IUPAC Name:
ammonium molybdate(VI)
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): ammonium molybdate(VI)
- Molecular formula: MoO4.2H4N
- Molecular weight: 196.0132 g/mol
- Smiles notation: [Mo](=O)(=O)([O-])[O-].[NH4+].[NH4+]
- InChl: 1S/Mo.2H3N.4O/h;2*1H3;;;;/q;;;;;2*-1/p+2
- Substance type: Inorganic
- Physical state: Solid
Specific details on test material used for the study:
- Name of test material: Ammonium molybdate
- Molecular formula: MoO42H4N
- Molecular weight: 196.0132 g/mol
- Substance type: Inorganic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Method

Target gene:
No data
Species / strain
Species / strain / cell type:
lymphocytes: Human
Details on mammalian cell type (if applicable):
No data
Additional strain / cell type characteristics:
not specified
Cytokinesis block (if used):
No data
Metabolic activation:
not specified
Metabolic activation system:
No data
Test concentrations with justification for top dose:
10 µM
Vehicle / solvent:
No data
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
METHOD OF APPLICATION: No data

DURATION
- Preincubation period: No data
- Exposure duration: 24 hrs
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: No data

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data

OTHER: No data
Rationale for test conditions:
No data
Evaluation criteria:
The slides were observed for induction of chromosome aberrations
Statistics:
No data

Results and discussion

Test results
Species / strain:
lymphocytes: Human
Metabolic activation:
not specified
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No data

Applicant's summary and conclusion

Conclusions:
Ammonium molybdate induced chromosome aberrations in human lymphocytes and hence is likely to be mutagenic in vitro.
Executive summary:

In vitro mammalian chromosome aberration test was performed to evaluate the mutagenic nature of ammonium molybdate. The study was performed at dose level of 10µM using human lymphocytes. The duration of exposure was 24 hrs. Ammonium molybdate induced chromosome aberrations in human lymphocytes and hence is likely to be mutagenic in vitro.