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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
data is from NTRL reports

Data source

Reference
Reference Type:
secondary source
Title:
Initial submission: The acute of toxicity certain compounds of molybdenum report to the International Molybdenum Association with attachments and cover letter dated 062891
Author:
International Molybdenum association
Year:
1991
Bibliographic source:
NTRL submission report -OTS0000811, last updated 1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
The ocular irritation potential of Ammonium molybdate was assessed in rabbits
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium molybdate(VI)
EC Number:
236-031-3
EC Name:
Ammonium molybdate(VI)
Cas Number:
13106-76-8
Molecular formula:
H4N.1/2MoO4
IUPAC Name:
ammonium molybdate(VI)
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): ammonium molybdate(VI)
- Molecular formula: MoO4.2H4N
- Molecular weight: 196.0132 g/mol
- Smiles notation: [Mo](=O)(=O)([O-])[O-].[NH4+].[NH4+]
- InChl: 1S/Mo.2H3N.4O/h;2*1H3;;;;/q;;;;;2*-1/p+2
- Substance type: Inorganic
- Physical state: Solid
Specific details on test material used for the study:
- Name of test material (as cited in study report): ammonium molybdate(VI)
- Molecular formula: MoO4.2H4N
- Molecular weight: 196.0132 g/mol
- Smiles notation: [Mo](=O)(=O)([O-])[O-].[NH4+].[NH4+]
- InChl: 1S/Mo.2H3N.4O/h;2*1H3;;;;/q;;;;;2*-1/p+2
- Substance type: Inorganic
- Physical state: Solid

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Sex: Male
- Source: A. Smith, Warlingham. Surrey
- Age at study initiation: 12 to 13 week
- Weight at study initiation: 2.8 to 3.1 kg
- Fasting period before study:
- Housing: The rabbits were individually housed in metal cages with perforated floors in Building
- Diet (e.g. ad libitum): SDS Standard Rabbit Diet
- Water (e.g. ad libitum):Tap water
- Acclimation period: The rabbits selected for the study were all acclimated to the laboratory environment

ENVIRONMENTAL CONDITIONS
- Temperature (°C):19 deg C
- Humidity (%):Relative humidity:30-70%
- Air changes (per hr): 19 air changes
per hour
- Photoperiod (hrs dark / hrs light): 12 hours of artificial light in each 24 hour period.

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes
Amount / concentration applied:
A 100 mg amount of Ammonium molybdate, the weight occupying a volume of 0.1 ml, was placed into the lower everted lid.
Duration of treatment / exposure:
single exposure
Observation period (in vivo):
Examination of the eyes was made after 1 hour and 1, 2, 3, 4 and.7 days after instillation.
Duration of post- treatment incubation (in vitro):
no data available
Number of animals or in vitro replicates:
6 male rabbits
Details on study design:
TEST SITE
- Area of exposure: lower everted lid.

SCORING SYSTEM: Grading and scoring of the ocular lesions were performed according to cornea,iris, Conjunctivae ,chemosis and redness in the eye of rabbit
Grading Scale for Eye Irritation:
Cornea Grade
Opacity: degree of density (readings should be taken from most dense area)*
No ulceration or opacity = 0
Scattered or diffuse areas of opacity (other than slight dulling of normal lustre); details of iris slightly obscured = 1*
Easily discernible translucent area; details of iris slightly obscured = 2*
Opalescent area; no details of iris visible; size of pupil barely discernible = 3*
Opaque cornea; iris not discernible through the opacity = 4*
Area of Cornea Involved
One quarter(or less) but not zero = 1
Greater than one quarter, but less than half = 2
Greater than half, but less than three quarters = 3
Greater than three quarters, up to whole area = 4
Iris
Normal = 0
Markedly deepened rugae, congestion, swelling, moderate circumcorneal hyperaemia; or injection; iris reactive to light (a sluggish reaction is considered to be an effect = 1 *
Hemorrhage, gross destruction, or no reaction to light = 2*

Conjunctivae
Redness (refers to palpebral and bulbar conjunctivae; excluding cornea and iris)
Vessels Normal = 0
Some blood vessels definitely hyperaemic (injected) = 1
Diffuse, crimson colour; individual vessels not easily discernible = 2*
Diffuse beefy red = 3 *
Chemosis
Swelling (refers to lids and/or nictating membranes)
No swelling = 0
Any swelling above normal (includes nictating membrane)= 1*
Obvious swelling, with partial eversion of lids = 2*
Swelling, with lids about half closed = 3*
Swelling, with lids more than half closed= 4*

*-interpreted as a positive effect (TSCA test criteria)

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 4 days
Score:
0
Reversibility:
fully reversible within: 4 days
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
All conjunctival reactions had resolved one to four days after instillation. No corneal or iridial inflammation was seen throughout the observation period . A diffuse crimson-red colouration of the conjunctivae was evident twenty-four hours after instillation only in one animal. Mild conjunctival inflammation was observed in the remaininq animals.

Any other information on results incl. tables

Ocular reactions elicited by Ammonium molybdate:

Rabbit no.

Region of eye

1 hr

Days after installation

1

2

3

4

7

1

Cornea

Density

0

0

0

0

0

0

Area

0

0

0

0

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

1

2

1

1

0

0

Chemosis

1

1

0

0

0

0

2

Cornea

Density

0

0

0

0

0

0

Area

0

0

0

0

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

1

1

1

1

0

0

Chemosis

1

0

0

0

0

0

3

Cornea

Density

0

0

0

0

0

0

Area

0

0

0

0

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

1

1

1

0

0

0

Chemosis

1

1

0

0

0

0

4

Cornea

Density

0

0

0

0

0

0

Area

0

0

0

0

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

1

1

0

0

0

0

Chemosis

1

0

0

0

0

0

5

Cornea

Density

0

0

0

0

0

0

Area

0

0

0

0

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

1

0

0

0

0

0

Chemosis

1

0

0

0

0

0

6

Cornea

Density

0

0

0

0

0

0

Area

0

0

0

0

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

1

0

0

0

0

0

Chemosis

1

0

0

0

0

0


Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
Instillation of Ammonium molybdate into the rabbit eyes elicited transient and very slight conjunctival inflammation only which were completely resolved after 4 days
The overall irritation scores after 7 days was 0.0
Based on the observations and scores, Ammonium molybdate can be considered not irritating to rabbit eyes.
Executive summary:

The ocular irritation potential of Ammonium molybdate was assessed in rabbits. The study was performed according to OECD 405 Guidelines. 100 mg of 0.1 ml chemical was installed into thelower everted lid of 6 New Zealand White male rabbits. The contralateral eye remained untreated and served as a control. Examination of the eyes was made after 1 hour and 1, 2, 3, 4 and.7 days after instillation. Observation of the eyes was aided by the use of a handheld torch. Grading and scoring of the ocular lesions were performed.

One of the animals gave a "positive" response. No corneal damage or iridial inflammation was seen throughout the observation period. A diffuse crimson-red colouration of the conjunctivae was evident twenty-four hours after instillation only in one animal. Mild conjunctival inflammation was observed in the remaininq animals. All conjunctival reactions had resolved one to four days after instillation.

Instillation of Ammonium molybdate into the rabbit eyes elicited transient and very slight conjunctival inflammation only which were completely resolved after 4 days

The overall irritation scores after 7 days was 0.0

Based on the observations and scores, Ammonium molybdate can be considered not irritating to rabbit eyes.