Registration Dossier
Registration Dossier
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EC number: 212-686-0 | CAS number: 846-48-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well documented GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Boldenone
- EC Number:
- 212-686-0
- EC Name:
- Boldenone
- Cas Number:
- 846-48-0
- Molecular formula:
- C19H26O2
- IUPAC Name:
- 17-hydroxyandrosta-1,4-dien-3-one
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Duration of test (contact time):
- 28 d
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 48
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 57
- Sampling time:
- 21 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- Boldenone (ZK5320) was degraded to 60% after 28 days. However, it is not ready biodegradable according to the stringetn criteria of the guidlein,
although significant degradation was observed. - Executive summary:
The testsubstance ZK 5320 was incubated in an aqueous solution including nutrients with microorganisms from a municipal sewage treatment plant for 28 days.
The biological degradation of the test and reference substances was evaluated by measurement of the O2consumption during the test period. O2consumption was continuously monitored and recorded by an automated device. The O2consumption was calculated as the percentage of total O2that the test material could theoretically have consumed, based on the molecular formula. The blank O2consumption was subtracted for correction.
In accordance with the OECD 301F(1), the test compound boldenone (ZK 5320) is not readily biodegradable under the conditions of the test and it was not toxic to the microbes of activated sludge. However, significant degradation up to 60 % occurred, but the 10 -day window was not passed.
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