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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-2-(dichloromethyl)benzene
EC Number:
201-849-1
EC Name:
1-chloro-2-(dichloromethyl)benzene
Cas Number:
88-66-4
Molecular formula:
C7H5Cl3
IUPAC Name:
1-chloro-2-(dichloromethyl)benzene
Details on test material:
Analytical Purity: > 99 %

Test solutions

Vehicle:
yes
Details on test solutions:
TWEEN 80 was used as dispersant for the test item

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Strain: Hamilton-Buchanan
- Source: West Aquarium Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (mean, range and SD): 2.8 cm, 2.5 - 3.4 cm, 0.19 cm
- Method of breeding: 22 +- 1 °C; O2-content >= 80% ; 12 h day/night cycle, 700 Lux; <= 1g fish/l water;
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period:14 days
- Acclimation conditions: same as test
- Type and amount of food:
- Food type: Tetra Min, Tetra Werke, Melle, Germany;
- Amount: ad libitum;
- Frequency: 2 times a day;

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
Test groups: 21.0 - 23.0 °C
Control groups: 21.2 - 23.0 °C

pH:
Test groups: 7.0 - 8.0
Control groups: 7.2 - 8.1

Dissolved oxygen:
Test groups: 4.6 - 9.4 mg/l
Control groups: 6.9 - 9.6 mg/l


Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
13.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 11.8 - 16.8
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
13.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 11.5 - 16.2
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.

Applicant's summary and conclusion

Conclusions:
From this study, LC0 (48 h and 96 h) for 2-chlorobenzal chloride was determined to be 10 mg/L, LC50 (48 h and 96 h) to be 13.9 mg/L and 13.5 mg/L and LC100 (48h and 96 h) to be 35 mg/L.