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EC number: 200-609-3 | CAS number: 65-45-2
Corneal opacity, permeability and in vitro irritation scores
mean corneal opacity
mean negative control corrected optical density
average group corrected optical density
A volume of 750 µL of salicylamide formulation, negative control (0.9% sodium chloride solution) or positive control (20% w/v imidazole) was applied to each of three excised bovine corneas followed by a 4 hour incubation at 32°C. After this incubation, each cornea was washed with media containing phenol red followed by media without phenol red, the opacities measured and then the anterior chamber emptied. For the permeability endpoint, sodium fluorescein solution was added into the anterior chamber and the corneas were incubated in the vertical position for 1.5 hours ± 5 minutes. Following this period, the media in the posterior chamber was removed and three 200 μL aliquots of this media (per cornea) were analysed for optical density at 490 nanometers.
The salicylamide formulation produced an In Vitro Irritation Score of 24.69.
The positive control article produced an In Vitro Irritation Score of 72.62.
Salicylamide was considered not to cause corrosion or severe irritation to the eye under the conditions of the assay.
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