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EC number: 944-329-6
CAS number: -
Table 1: Mobility of Daphnia magna after 24 and 48 h
Loading rate [mg/L]
No of Daphnids
Immobilized Daphnids after 24 h
Immobilized Daphnids after 48 h
Table 2: Measured concentrations of WAF loading rates
Sampling Day/Sample Age [d/h]
WAF loading rate [mg/L]
Measured concentration [mg/L]
Sample preparation factor
Determined concentration of test item [mg/L]
% of initially measured concentration
n.d. = no test item detected
n.a. = not applicable
* Estimated value, the determined concentration was below the LOQ.
LOQ: 0.115 mg/L
Table 3: Validity criteria
Criterion from the guideline
Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more that 10% of the daphnids should have been immobilized.
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.
8.4 - 8.7 mg O2/L
EL50 (48 h): 89 mg/L (nominal, OECD 202)
One experimental study is available investigating the short-term
toxicity of the test item to aquatic invertebrates. The acute
toxicity of the test item to Daphnia magna was determined in a 48-hour
static test according to OECD 202 (2004) under GLP conditions. Since the
test item is a complex mixture (UVCB) consists of several constituents
with differently low solubility, Water Accommodated Fractions (WAFs)
with the loading rates of 6.25, 12.5, 25, 50 and 100 mg/L were tested to
assess the toxicity of the test item to daphnids. Additionally, a
control group (test water without test item) was tested in parallel. The
actual test concentrations in the test medium of the two highest loading
rates were verified by GC/FID measurement at the start and end of the
test. At the start of the test, the measured concentrations of
the analyzed components of the test item in the test media with loading
rates of 50 and 100 mg/L were 0.51 and 0.94 mg/L, respectively. The
measured concentrations at the end of the test were in the range of 13
and 16 % of the initially measured values. The 48-hour EL50 was
calculated (based on loading rates) to be 89 mg/L. Since WAFs were
tested, all reported biological results were based on the loading rates
of the test item. The preparation of the WAFs and procedure of
evaluation of data of WAFs is based on recommendations of the OECD
Guidance Document No. 23 on Aquatic Toxicity Testing of Difficult
Substances and Mixtures, 2000.
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