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EC number: 945-942-1
CAS number: -
Acceptability of the Test
- In the control, cell density increased by an average factor of at
least 16 within the exposure period (i.e. 298).
- The mean coefficient of variation for section-by-section specific
growth rates in the control cultures did not exceed 35 % (i.e. 15 %).
- The coefficient of variation of average specific growth rates during
the whole test period in replicate control cultures did not exceed 7 %
(i.e. 1.4 %).
Table 1: Summary of results for the total test period
Test material concentration
100 (13 mg/L)
* - effect was statistically significant
Table 2: Growth Rate And Percentage Inhibition At Different
The objectiveofthe study was to evaluate the test material for its
ability to generate toxic effects in Pseudokirchneriella subcapitata during
an exposure period of 72 hours and, if possible, to determine the NOEC,
EC10and EC50for both inhibition of growth rate and
inhibition of yield.The study was performed in accordance with the
standardised guideline OECD 201, under GLP conditions.
A Saturated Solution (SS) of the test
material was prepared at a loading rate of 100 mg/L and used as the
highest concentration. Lower concentrations were prepared by diluting
the highest concentration in test medium.
A combined limit/range-finding test was performed. Six
exponentially growing algal cultures per group were exposed to an
untreated control and to solutions containing 1.0, 10 and 100 % of the
SS prepared at a loading rate of 100 mg/L. The initial algal cell
density was 10^4cells/mL.The total exposure period was 72
hours and samples for analytical confirmation of actual exposure
concentrations were taken at the start, after 24 and 72 hours of
No biologically significant differences were recorded between the
values for growth rate at any of the test concentrations when compared
to the control group.
Samples taken from the undiluted SS were analysed. The results
were, however, not acceptable since they were above the validated range.
Consequently, the reserve samples were analysed. The actual exposure
concentration was 13 mg/L at the start of the test. This concentration
remained stable throughout the test duration, i.e. was 101 % of initial
during the test. Based on this results, effects parameters were based on
the initially measured concentration.
Under the conditions of this study with Pseudokirchneriella subcapitata,
the test material showed no biologically relevant inhibition of growth
rate at any of the concentrations tested. Both the EC50 for growth rate
inhibition (72h-ERC50) and the EC50 for yield inhibition (72h EYC50)
were beyond the range tested, i.e. exceeded 13 mg/L, being considered
the maximum soluble concentration of test material in test medium at a
loading rate of 100 mg/L. The 72h-NOEC for growth rate inhibition was 13
mg/L based on biologically relevance. The 72h-NOEC for yield inhibition
was lower than 13 mg/L based on both statistically significance and
The objective of the study was to evaluate the test material for
its ability to generate toxic effects inPseudokirchneriella
subcapitataduring an exposure period of 72 hours and, if possible,
to determine the NOEC, EC10and EC50for both
inhibition of growth rate and inhibition of yield. The study was
performed in accordance with the standardised guideline OECD 201, under
GLP conditions. The study was awarded a reliability score of 1 in
accordance with the criteria set forth by Klimisch et al. (1997).
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