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EC number: 235-460-3 | CAS number: 12236-25-8
Endpoint summary
Administrative data
Description of key information
Short term toxicity to aquatic invertebrate:
Toxicity to aquatic invertebrates of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 16.88 mg/L when exposed to Daphnia magna for 48 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
Toxicity to aquatic algae and cynobacteria :
Toxicity to aquatic algae and cynobacteria of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 36.36 mg/L when exposed to Desmodesmus subspicatus for 72 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria
Additional information
Short term toxicity to aquatic invertebrate:
Toxicity to aquatic invertebrates of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 16.88 mg/L when exposed to Daphnia magna for 48 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
The above prediction was supported by experimental data conducted by W.E Bishop, R.D Cardwell, B.B Heidolph summarized in Aquatic toxicology and hazard assessment : Sixth symposium , W.E Bishop, R.D Cardwell, B.B Heidolph. 1981. for structrually similar read across substance 619-72-7 (4-nitrobenzonitrile)
4-nitrobenzonitrile when subjected to Daphnia magna under static condition for 48h The EC50 value was considered to be 49.3mg/l
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
The above experimental data was further supported by experimantal result summarized in Assessment for the Challenge Propanenitrile, 3-[ethyl[3-methyl-4-[(6-nitro-2-benzothiazolyl)azo]phenyl]amino]- (Disperse Red 179) Environment Canada Health Canada 2010 for structurally similar read across substance 16889-10-4 (2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile).
2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile when subjected on Daphnia for 48 hrs the effect concentration (EC50) was observed as 23 mg/l.
Based on the above data for the Target as well as the read across substance and by applying weight of evidence
the substance
2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
Toxicity to aquatic algae and cynobacteria :
Toxicity to aquatic algae and cynobacteria of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 36.36 mg/L when exposed to Desmodesmus subspicatus for 72 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
The above prediction was suppportted by experimental data summarized in Assessment for the Challenge Propanenitrile, 3-[ethyl[3-methyl-4 -[(6-nitro-2-benzothiazolyl) azo]phenyl]amino]- (Disperse Red 179) Environment Canada Health Canada 2010 for the structurally similar read across substance 16889-10-4 (2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile).
2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile when subjected on Scenedesmus subspicatus for 72 hrs the effect concentration (EC50) was observed as >10 mg/l.
Another structurally similar read across substance was summarized in the same referance 31482-56-1 (3-[ethyl[4-[(4-nitrophenyl) azo]phenyl]amino]propiononitrile).
3-[ethyl[4-[(4-nitrophenyl)azo]phenyl]amino]propiononitrile when subjected on Scenedesmus subspicatus for 72 hrs the effect concentration (EC50) was observed as 52 mg/l.
Based on the above data for the Target as well as the read across substance and by applying weight of evidence
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