Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 235-460-3 | CAS number: 12236-25-8
Endpoint summary
Administrative data
Description of key information
Short term toxicity to aquatic invertebrate:
Toxicity to aquatic invertebrates of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 16.88 mg/L when exposed to Daphnia magna for 48 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
Toxicity to aquatic algae and cynobacteria :
Toxicity to aquatic algae and cynobacteria of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 36.36 mg/L when exposed to Desmodesmus subspicatus for 72 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria
Additional information
Short term toxicity to aquatic invertebrate:
Toxicity to aquatic invertebrates of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 16.88 mg/L when exposed to Daphnia magna for 48 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
The above prediction was supported by experimental data conducted by W.E Bishop, R.D Cardwell, B.B Heidolph summarized in Aquatic toxicology and hazard assessment : Sixth symposium , W.E Bishop, R.D Cardwell, B.B Heidolph. 1981. for structrually similar read across substance 619-72-7 (4-nitrobenzonitrile)
4-nitrobenzonitrile when subjected to Daphnia magna under static condition for 48h The EC50 value was considered to be 49.3mg/l
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
The above experimental data was further supported by experimantal result summarized in Assessment for the Challenge Propanenitrile, 3-[ethyl[3-methyl-4-[(6-nitro-2-benzothiazolyl)azo]phenyl]amino]- (Disperse Red 179) Environment Canada Health Canada 2010 for structurally similar read across substance 16889-10-4 (2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile).
2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile when subjected on Daphnia for 48 hrs the effect concentration (EC50) was observed as 23 mg/l.
Based on the above data for the Target as well as the read across substance and by applying weight of evidence
the substance
2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
Toxicity to aquatic algae and cynobacteria :
Toxicity to aquatic algae and cynobacteria of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 2-[[4-[bis(2-hydroxyethyl)amino]-o-tolyl]azo]-5-nitrobenzonitrile was estimated to be 36.36 mg/L when exposed to Desmodesmus subspicatus for 72 hrs.
This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.
The above prediction was suppportted by experimental data summarized in Assessment for the Challenge Propanenitrile, 3-[ethyl[3-methyl-4 -[(6-nitro-2-benzothiazolyl) azo]phenyl]amino]- (Disperse Red 179) Environment Canada Health Canada 2010 for the structurally similar read across substance 16889-10-4 (2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile).
2-[[4-[(2-cyanoethyl)ethylamino]phenyl]azo]-5-nitrobenzonitrile when subjected on Scenedesmus subspicatus for 72 hrs the effect concentration (EC50) was observed as >10 mg/l.
Another structurally similar read across substance was summarized in the same referance 31482-56-1 (3-[ethyl[4-[(4-nitrophenyl) azo]phenyl]amino]propiononitrile).
3-[ethyl[4-[(4-nitrophenyl)azo]phenyl]amino]propiononitrile when subjected on Scenedesmus subspicatus for 72 hrs the effect concentration (EC50) was observed as 52 mg/l.
Based on the above data for the Target as well as the read across substance and by applying weight of evidence
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
