trans-4-ethyl-4'-(4-propylcyclohexyl)-1,1'-biphenyl

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-09-29 to 2010-12-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study performed according to OECD 305 and METI Guideline: Concentration Test on Chemical Substances in Fish.

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Qualifier:

according to guideline

Guideline:

other: METI Guideline: Concentration Test on Chemical Substances in Fish

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Puritiy: > 99.94 %

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals/frequency for test medium samples: days 0, 4, 8, 14, 21, 28

Vehicle:

yes

Details on test solutions:

According to guideline

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 10 mg/l

Dose 1: Test item: 0.001 mg/L ,
Dose 2: Test item: 0.0001 mg/L

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Cyprinus carpio
Lot: 100215c
Weight: 7.5 g
Length: 8.5 cm

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Hardness:

250 g/L

Test temperature:

25.0 ° +/- 0.4 °C

pH:

7

Dissolved oxygen:

saturated

Nominal and measured concentrations:

nominal concentration 0.0010 mg/L:
Solubilizer used: HCO-40, 10 mg/L

analytically measured concentrations:
day 4: 0.000918 mg/L
day 7: 0.000962 mg/L;
day 14: 0.000972 mg/L
day 21: 0.000970 mg/L
day 28: 0.000990 mg/L


nominal concentration 0.0001 mg/L
Solubilizer used: HCO-40, 10 g/L
analytically measured concentrations:
day 4: 0.000101 mg/L
day 7: 0.000102mg/L;
day 14: 0.000100 mg/L
day 21: 0.0000998mg/L
day 28: 0.0000988 mg/L

Details on test conditions:

After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 4, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.001 and 0.0001 mg/L were maintained during the experimental phase.

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

0.001 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Concentrations maintained using HCO-40

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

0.001 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Concentrations maintained using HCO-40

Validity criteria fulfilled:

yes

Conclusions:

The test material showed no toxic potential at concentrations at or above the water solubility. The NOEC was 0.001 mg/L and thus higher than the limit of water solubility.

Executive summary:

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. During the GLP compliant test the lethal and other effects in fish were determined during the exposure period of 28 days.

The test material showed no toxic potential at concentrations at or above the water solubility. The NOEC was 0.001 mg/L and thus higher than the limit of water solubility.

Description of key information

Data from a GLP study according to OECD TG 305 are available.

NOEC (28 d) = 0.001 mg/L (10 mg/L HCO-40)

LC50 (28 d)  > 0.001 mg/L (10 mg/L HCO-40)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.2 mg/L

Additional information

NOEC (BCF, 28d) > 0.20 mg/L