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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
no
Remarks:
The GLP audit of the Laboratory was in process (by MAGYOSZ)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
Test item: N-(2-HYDROXYETHYL) PHTHALIMIDE
CAS No.: 3891-07-4
Physical state: solid, powder
Colour and Odour: white, odourless
Active Ingredient Content (TLC): 98.95 %
Storage: on dry place, protected from light, 15-30 °C

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
The numbers of immobilised daphnids were counted at 24 and 48 hours after the start of test.

Test solutions

Vehicle:
yes
Details on test solutions:
The test organism was breaded and hold in Elendt M4 medium which was used also for preparing of test item stock and working solutions.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Test species: Daphnia magna Straus
Young daphnids were used in the test should have been less than 24 hours old and were not first brood progeny.
Age: < 24 hours neonates
Source: National Institute of Environmental Health

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
21 ± 1 °C
pH:
The pH was measured at the beginning and at the end of test. of test.
Dissolved oxygen:
The oxygen concentrations was measured at the beginning and at the end of test.
Nominal and measured concentrations:
The following concentrations were applied: 50.0; 100.0; 200.0; 300.0; 400.0; 600.0 and 800.0 mg/l.
The evaluation of the effects was based on the nominal concentration of test item.
Details on test conditions:
At each concentration level four replicates were used. Each replicate contained 5 daphnids in 50 ml test solution. The test vessels were covered with plastic lid and were kept in thermostat at 21 ± 1 °C with a 16/8 hours light/dark cycle. The treatment of control vessels was same as test vessels.
The pH and oxygen concentrations were measured at the beginning and at the end of test. The numbers of immobilised daphnids were counted at 24 and 48 hours after the start of test.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
190.17 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
413.17 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL
Details on results:
The statistical analyses of data were performed by ToxRat® software.
The NOEC and LOEC were determined with Fisher’s exact binominal test with Bonferroni correction. The results were inconclusive.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this Daphnia magna, Acute Immobilisation Test for N-(2-Hydroxyethyl) Phthalimide (CAS No. 3891-07-4) the results indicated that the test item is not hazardous to the aquatic environment.