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Diss Factsheets
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EC number: 946-742-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 - 13 Nov 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The study was conducted according to the appropriate OECD test guideline and in compliance with GLP. Test solution described as cloudy grey WAF and effects reported are well above the water solubility of the test substance. No analytical verification was conducted. Thus effects might be overestimated and being physical effects.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-[3-(dimethylamino)propyl] amides C18-22 (even numbered)
- IUPAC Name:
- N-[3-(dimethylamino)propyl] amides C18-22 (even numbered)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 100 mg/L was prepared and stirred for 20 hours and left to settle for four hours to produce a cloudy grey water accomodated fraction. The test concentrations were prepared by addition of the appropriate volume of the stock solution using pipettors to test media. They were then made up to the mark in volumetric flasks to give the required concentrations for the test.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: CEFAS Laboratories, Lowestoft
- Age at study initiation (mean and range, SD): less than 24 hours
- Culture conditions: Temperature: 20 ± 2 °C; Dissolved oxygen: >60% ASV; Photoperiod: 16 hours light and 8 hours dark
- Feeding during test: none
- Feeding regime: Each working day, a suspension of Chlorella vulgaris at a rate of 1 mg organic carbon / litre of culture water until 24 hours before the test initiation
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.8 - 21.1 °C
- pH:
- 7.4 - 7.8
- Dissolved oxygen:
- 7.3 - 8.7 mg O2/L
- Nominal and measured concentrations:
- control, 10, 18, 32, 56 and 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 60 mL plastic vessels, filled with 25 mL test solution
- Aeration: before start
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Test and culture medium were the same: reconstituted ISO water with a hardness of 156 mg/L CaCO3. The temperature was adjusted to 18 - 22 °C, aerated until the dissolved oxygen concentration had reached a minimum of 60% air saturation and the pH stabilised between 6 and 9
- Intervals of water quality measurement: pH and dissolved oxygen determinations on pooled replicates for each test and control solution were carried out immediately prior to initiating the test and at the end of the 48 hour test period, temperature was recorded at 0, 24 and 48 hours.
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16 hours light, 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilisation at 24 and 48 hrs
TEST CONCENTRATIONS
- Range finding study:
Test concentrations: control, 0.1, 1, 10, and 100 mg/L
Results used to determine the conditions for the definitive study: Data identified the 48 hour EC50 as being between 10 and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- 74.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 65.1 to 80.3
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 43.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 40.1 to 44.4
- Results with reference substance (positive control):
- EC50 (48 h) = 0.27 mg/L
Any other information on results incl. tables
Table 1: Results range-finding test
Exposure period (hours) |
Cumulative percentage immobilisation |
||||
Concentration mg/L |
|||||
0 |
0.1 |
1 |
10 |
100 |
|
24 |
0 |
0 |
0 |
0 |
5 |
48 |
0 |
0 |
0 |
0 |
5 |
Table 2: Cumulative immobilisation definitive test
Concentration mg/L |
Number immobilised |
% immobilisation |
||
24 hours |
48 hours |
24 hours |
48 hours |
|
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
18 |
0 |
0 |
0 |
0 |
32 |
0 |
1 |
0 |
5 |
56 |
3 |
20 |
15 |
100 |
100 |
20 |
20 |
100 |
100 |
Table 3: Observations definitive test
Concentration mg/L |
Recorded observations |
|
24 hours |
48 hours |
|
0 |
20 NS |
20 NS |
10 |
20 NS |
20 NS |
18 |
20 NS |
20 NS |
32 |
20 NS |
2 MS, 17 NS, 1 I |
56 |
17 NS, 3 I |
20 I |
100 |
20 I |
20 I |
MS = movement slower, NS = no signs of abnormalities, I = immobilised
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.