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Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jun 30 - Aug 16, 2004
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Use of solvents to increase exposure; Pre test for bioaccumulation study. This study was performed according to GLP and the methods applied are fully compliant with OECD TG 203.
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
Details on sampling:
0; 24, 48, 72, 96 h
Details on test solutions:
According to guideline PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): SPAN 60
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)):

Dose 1: Positive control: 0.5 mg/L , Vehicle: 500 mg/L
Dose 2: Test item: 0.05 mg/L , Vehicle: 500 mg/L
Control: Test item: --, Vehicle: 500 mg/L
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Weight: 0.31 g
Length: 3.4 cm
Lot: 40930
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Post exposure observation period:
250 mg/L
Test temperature:
24.7 +/- 0.2 °C
Dissolved oxygen:
7.1 mg/L
Details on test conditions:
10 fish per 4 L
Reference substance (positive control):
not specified
96 h
Dose descriptor:
Effect conc.:
100.9 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Mortality of control:
0 % after 96 hours

Mortality in %
Test material (mg/L) 24 h 48 h 72 h 96 h
100.9 mg/L 0 0 0 0
SPAN 60 (500 mg/L) 0 0 0 0
Control Group (Vehicle: 500 mg/L): no mortality observed during the study

- Effect concentrations exceeding solubility of substance in test medium:
All concentrations tested were at or above the solubility limit of the pure test substance.

To achive the exposure concentrations, solvent SPAN 60 was used.
Validity criteria fulfilled:
The NOEC of the test material with solubilizer is at 100.9 mg/L, which is above the limit of water solubility.

Description of key information

This study was performed according to GLP and the methods applied are fully compliant with OECD TG 203. Solubilizer has been applied to achieve maximum exposure.

The NOEC ammounts to 100.9 mg/L and the LC50 of the test material is clearly above the limit of water solubility.

Key value for chemical safety assessment

Additional information

The LC50 was larger than 100.9 mg/L. The NOEC was at 100.9 mg/L.