Essential oil of Cistus ladaniferus L (Cistaceae) obtained from stems and leaves by distillation

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 15 to 19 Septembre 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

GLP compliance programme (inspected on 13-14 October 2014 / signed on 8 April 2015)

Type of method:

method according to Siwoloboff

Key result

Boiling pt.:

ca. 190.4 °C

Atm. press.:

ca. 101 325 Pa

Decomposition:

no

Remarks:

After cooling down a slightly change of the colour was observed.

Remarks on result:

other:

Remarks:

Mean ± standard deviation (after correction following Sydney Young) = 190.4 ± 0.3 °C (463.5 ± 0.3 K) at standard pressure (101.325 kPa).

The measured values and observations are presented in the following tables.

Table 4.3.1    Results

Determination	Boiling Point	Atmospheric Pressure
First determination	190.0 °C (463.2 K)	99787 Pa
Second determination	189.6 °C (462.8 K)	99759 Pa

 

Table 4.3.2    Observations

Observations	Temp. in °C First Determination	Temp. in °C Second Determination
When setting the sample tube into an aluminium block came now and then bubbles, after that the bubbles disappeared.	176.2	176.2
Bubble chain, heating plate turned off	203.2	201.4

 

After cooling down a slightly change of the colour was observed.

 Table 4.3.3    Corrected values following Sidney-Young

	Boiling point	Pressure in Pa	Corrected Boling point
First determination	190.0 °C (463.0 K)	99787 Pa	190.59 °C (463.74 K)
Second determination	189.6 °C (462.8 K)	99757 Pa[SRD1]	190.20°C (463.35 K)
Mean			190.4 °C (463.5 K)

 

As the difference between the two determinations was below 2 K the result can be considered valid and theresult is calculated as mean of the two values of the duplicate determination.

Mean ± standard deviation (after correction following Sydney Young) = 190.4 ± 0.3 °C (463.5 ± 0.3 K) at standard pressure (101.325 kPa).

Conclusions:

The initial boiling temperature of the test item has been determined to be ca 190.4± 0.3 °C, corrected to standard pressure (101.325 kPa).

Executive summary:

The initial boiling point of the test item was measured under GLP according to OECD 103 / EU A2 guideline, by Siwoloboff.

The test item was filled into a sample tube. The sample tube was then set into the aluminium block at 176.2 °C (first and second determination). For the increase of the temperature, a temperature gradient of 1 °C /min was maintained. When bubbles rose constantly on the capillary, heating was turned down, and, cooling slowly. The temperature at which the chain of bubbles broke was recorded (with its atmospheric pressure) and is considered as the boiling point.The determination was performed in duplicate.

After cooling down a slightly change of the colour was observed.

Description of key information

The initial boiling point of the test item has been determined to be ca 190.4 ± 0.3 °C, corrected to standard pressure (101.325 kPa).

Key value for chemical safety assessment

Boiling point at 101 325 Pa:

190.4 °C

Additional information

A fully reliable experimental study, conducted according to a recognized OECD/EC method and under GLP, is available. It is considered as a key study.