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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2013-04-09- 2013-04-11
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
The acute immobilization study on Daphnia magna was performed according to the OECD Guideline for the Testing of Chemicals No. 202 (2004) [1] and the SOP/W/21.
The preliminary tests were performed to determine the range of concentrations to be used in the definitive test.
The tests were performed using the Elendt M7 medium as a diluent necessary to prepare the test concentrations. The medium was aerated prior to the beginning of the test. The daphnids were exposed in glass beakers with a capacity of 150 mL. The beakers were covered with transparent lids in order to minimize evaporation and to prevent accidental contamination. In the preliminary tests, the volume of the test concentration and the test medium for the control in every replicate was 50 mL. In the definitive test, the volume of the test each concentration and the test medium for the control in every replicate was 100 mL. Five individuals of Daphnia magna were used in each replicate; hence, there were 10 or 20 mL of the test solution per daphnid (the OECD Guideline No. 202 requires at least 2 mL) [1]. Each test concentration and the control were tested in four replicates. The daphnids were exposed for 48 hours. The tests were conducted in static design.
During the exposure, the daphnids were not fed. The tests were performed under a 16-hour light and 8-hour dark regime.
Test organisms (species):
Daphnia magna
Details on test organisms:
The test organism, Daphnia magna Straus originated from the standard laboratory culture cultivated at the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology [SOP/W/67]. Only organisms up to 24 hours old (not first brood progeny) and in good physiological condition were used in the experiments. The sensitivity of the culture was monitored on a regular basis using a reference substance, potassium dichromate.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
21.0 – 22.7 ° C
pH:
pH of the control: 7.23 – 7.27
Dissolved oxygen:
oxygen concentration in the control: 8.4 – 8.5 mg/L
Nominal and measured concentrations:
The WAFs prepared from a loading of 6.25, 12.5, 25, 50 and 100 mg/L used in the definitive test were determined based on the preliminary tests results.
In samples of fresh solutions the determined concentrations of the total organic carbon in the control and in the WAF of a loading of 6.25 mg/L and 12.5 mg/L were below LoQ value, in the WAF of a loading of 25 mg/L was 1.186 mg C/L, in the WAF of a loading of 50 mg/L was 1.375 mg C/L and in
the WAF of a loading of 100 mg/L was 2.340 mg C/L.
In the samples of 48-hour-old solutions, the determined concentrations of the total organic carbon in control and in the WAF of a loading of 6.25 mg/L and 12.5 mg/L were below LoQ value, in the WAF of a loading of 25 mg/L was 1.178 mg C/L, in the WAF of a loading of 50 mg/L was 1.508 mg C/L and in the WAF of a loading of 100 mg/L was 2.464 mg C/L.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
55.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
The substance was tested according to OECD 202. After 48h the EC50 was determined to be EC50:55.7 mg/l.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Being a tridodecylamine and a tri-C8,10-amine, target and source substance are structurally very similar only differing slightly in the length of the alkyl chain. Thus, both target and source subtance have identical functional group(s), common precursor(s)/breakdown product(s) as well as common mechanism(s) of action.
Reason / purpose:
read-across source
Vehicle:
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
55.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Conclusions:
Based on data for a suitable read across substance, one can assume that Tridodecylamine is toxic to aquatic invertebrates.

Description of key information

EC50(48h) = 55.7 mg/L (nominal) for immobilisation of Daphnia magna (OECD 202, read across)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
55.7 mg/L

Additional information