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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Link to relevant study record(s)

Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000-09-14 to 2000-12-27
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
other: METI Guideline: Concentration Test on Chemical Substances in Fish
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Batch: E998833

Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 28, 35, 42, 49, and 60
- Sampling intervals/frequency for test medium samples: days 0, 7, 14, 21, 28, 35, 42, 49, and 60
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):
Water: Extraction with CH2Cl2 with further chromatographic analysis.
Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane.
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
According to guideline
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)):
Test solution 1: 0.02 mg/L mg/L test material, 0.4 mg/L HCO-40
Test solution 2: 0.002 mg/L test material, 0.04 mg/L HCO-40
Control: 0.4 mg/L HCO-40
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Weight: 5 g
Length: 8 +/- 4 cm
Lot: 00-K-0630
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
60 d
Total depuration duration:
0 d
Test temperature:
23.4-24.2°C
pH:
7.2-7.7
Dissolved oxygen:
7.1-7.5 mg/L
Salinity:
According to guideline
Nominal and measured concentrations:
nominal concentration
0.02 mg/L:
analytically measured concentrations:
day 7: 0.0203 mg/L;
day 14: 0.0201 mg/L
day 21: 0.0204 mg/L
day 28: 0.0202 mg/L
day 35: 0.0203 mg/L
day 42: 0.0202 mg/L
day 49: 0.0199 mg/L
day 60: 0.0198 mg/L

nominal concentration
0.002 mg/L:
analytically measured concentrations:
day 7: 0.00179 mg/L;
day 14: 0.00182 mg/L
day 21: 0.00182 mg/L
day 28: 0.00186 mg/L
day 35: 0.00189 mg/L
day 42: 0.00191 mg/L
day 49: 0.00189 mg/L
day 60: 0.00189 mg/L
Details on estimation of bioconcentration:
according to guideline
Key result
Conc. / dose:
0.02 mg/L
Temp.:
24 °C
pH:
7.2
Type:
BCF
Value:
276 dimensionless
Basis:
normalised lipid fraction
Remarks on result:
other: analytical concentration
Remarks:
Conc.in environment / dose:0.0005 mg/L
Key result
Conc. / dose:
0.002 mg/L
Temp.:
24 °C
pH:
7.2
Type:
BCF
Value:
664 dimensionless
Basis:
normalised lipid fraction
Remarks on result:
other: analytical concentration
Remarks:
Conc.in environment / dose:0.00005 mg/L
Details on kinetic parameters:
Due to the low uptake, no depuration phase was considerd necessary.
Metabolites:
none
Details on results:
- Mortality of test organisms: no effects
- Behavioural abnormalities:no effects
- Observations on body length and weight:no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects
Validity criteria fulfilled:
yes
Conclusions:
The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 276 and 664 (mean 428).

Description of key information

The informtion for this endpoint study record was obtained from an experimental study.

The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 305.

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 276 and 664 (mean 428).

Key value for chemical safety assessment

BCF (aquatic species):
428 dimensionless

Additional information