4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino]-2-sulphophenyl]azo]-5-hydroxynaphthalene-2,7-disulphonic acid

Endpoint summary

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

Gene mutation in vitro:

Ames assay:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, gene mutation was predicted for 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino] -2-sulphophenyl]azo]-5-hydroxynaphthalene  -2,7-disulphonic acid. The study assumed the use of Salmonella typhimurium strains TA 1535, TA 1537, TA 98, TA 100 and TA 102 with S9 metabolic activation system. 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino] -2-sulphophenyl]azo] -5- hydroxynaphthalene  -2,7-disulphonic acid was predicted to not induce gene mutation in Salmonella typhimurium strains TA 1535, TA 1537, TA 98, TA 100 and TA 102 in the presence of S9 metabolic activation system and hence, according to the prediction made, it is not likely to classify as a gene mutant in vitro.

Based on the predicted result it can be concluded that the substance is considered to not toxic as per the criteria mentioned in CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (negative)

Genetic toxicity in vivo

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Gene mutation in vitro:

Prediction model based estimation for the target chemical and data from target and read across chemicals have been reviewed to determine the mutagenic nature of 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino] -2-sulphophenyl]azo]-5-hydroxynaphthalene  -2,7-disulphonic acid. The studies are as mentioned below:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, gene mutation was predicted for 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino] -2-sulphophenyl]azo]-5-hydroxynaphthalene  -2,7-disulphonic acid. The study assumed the use of Salmonella typhimurium strains TA 1535, TA 1537, TA 98, TA 100 and TA 102 with S9 metabolic activation system. 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino] -2-sulphophenyl]azo] -5- hydroxynaphthalene  -2,7-disulphonic acid was predicted to not induce gene mutation in Salmonella typhimurium strains TA 1535, TA 1537, TA 98, TA 100 and TA 102 in the presence of S9 metabolic activation system and hence, according to the prediction made, it is not likely to classify as a gene mutant in vitro.

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, chromosomal aberration was predicted for 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl] amino]-2-sulphophenyl]azo]-5-hydroxynaphthalene  -2,7-disulphonic acid. The study assumed the use of Chinese hamster ovary (CHO) cell line with and without S9 metabolic activation system. 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino]-2-sulphophenyl]azo]-5- hydroxynaphthalene  -2,7-disulphonic acid was predicted to not induce chromosomal aberrations in Chinese hamster ovary (CHO) cell line in the presence and absence of S9 metabolic activation system and hence, according to the prediction made, it is not likely to classify as a gene mutant in vitro.

The ability of 4-amino-3,6-bis[(E)-2-[4-({4-chloro-6-[(3-sulfophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfophenyl]diazen-1-yl]-5-hydroxynaphthalene-2,7-disulfonic acid to induce chromosomal aberration was predicted using Chinese hamster ovary cells (CHO) using Danish QSAR database (2017). The end point for chromosome aberrations has been modeled in the Danish QSAR using the three software systems Leadscope, CASE Ultra and SciQSAR. Based on predictions from these three systems, a fourth and overall battery prediction is made. The battery prediction is made using the so called Battery algorithm. With the battery approach it is in many cases possible to reduce “noise” from the individual model estimates and thereby improve accuracy and/or broaden the applicability domain. 4-amino-3,6-bis[(E)-2-[4-({4-chloro-6-[(3-sulfophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfophenyl]diazen-1-yl]-5-hydroxynaphthalene-2,7-disulfonic acid does notinduce chromosome aberrations inChinese hamster ovary cells (CHO)and hence is predicted to not classify as a gene mutant in vitro.

The predicted data is further supported by the data from target chemical and its read across chemicals as mentioned below:

In a study by Venturini and Tamaro (Mutation Research, 1979), Bacterial gene mutation test was performed to evaluate the mutagenic response for 50 -60% structurally and functionally similar read across chemical Red H3B (C.I. reactive red 3; RA CAS no 23211 -47 -4; IUPAC name: trisodium 5-{[4-chloro-6-(phenylamino)-1,3,5-triazin-2-yl]amino}-4-hydroxy-3-[(E)-2-(2-sulfonatophenyl)diazen-1-yl]naphthalene-2,7-disulfonate). The test was performed using Salmonella typhimurium strainsTA1535, TA100, TA1538, and TA98 in the presence and absence of S9 metabolic activation system. The test compound was dissolved in DMSO and used at dose levels of 100, 500 or 1000µg/plate. Concurrent positive control chemicals were also included in the study. Xylene light yellow 2G (C.I. acid yellow 17)did not induce reversion of mutation when applied to Salmonella typhimurium strainsTA1535, TA100, TA1538, and TA98 in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant in vitro.

In another study by Joachim et al (Mutation Research, 1985), Salmonella/microsome assay was performed to evaluate the mutagenic response for another 50 -60% structurally and functionally similar read across chemical Food black 2 (FB2; RA CAS no 2118 -39 -0; IUPAC name: tetrasodium (3E)-6-amino-4-oxo-3-(2-{7-sulfinato-4-[(E)-2-(4-sulfonatophenyl)diazen-1-yl] naphthalen-1-yl}hydrazin-1-ylidene)-3,4- dihydronaphthalene-2,7-disulfonate). The test was performed using Salmonella typhimurium strains TA1538 and TA98 in the presence and absence of S9 metabolic activation system upto a dose level of 5 mg/plate. The test chemical was dissolved in DMSO and a dose-related increase (at least 2-fold) in revertant colonies was used to define a significant mutagenic response. Food black 2 (FB2)did not induce reversion of mutation when applied to Salmonella typhimurium strains TA1538 and TA98 in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant in vitro.

Based on the data available for the target chemical and its read across, 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino] -2-sulphophenyl]azo]-5-hydroxynaphthalene  -2,7-disulphonic acid does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant as per the criteria mentioned in CLP regulation

Justification for classification or non-classification

Based on the data available for the target chemical and its read across, 4-amino-3,6-bis[[4-[[4-chloro-6-[(3-sulphophenyl)amino]-1,3,5-triazin-2-yl]amino] -2-sulphophenyl]azo]-5-hydroxynaphthalene  -2,7-disulphonic acid (CAS no 51357 -74 -5) does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant as per the criteria mentioned in CLP regulation.