1,2,3,4,4a,7,8,8a-octahydro-2,4a,5,8a-tetramethyl-1-naphthyl formate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

The study was conducted in 1978.

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Documentation insufficient for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

A single application of the test material was added to 2 cm2 of clipped skin on the flank of a guinea pig. Skin reactions were noted at 24 hours.

GLP compliance:

no

Remarks:

Study pre-dates GLP.

Test material

Test material information

Constituent 1

Reference substance name:

1,2,3,4,4a,7,8,8a-octahydro-2,4a,5,8a-tetramethyl-1-naphthyl formate

EC Number:

265-742-1

EC Name:

1,2,3,4,4a,7,8,8a-octahydro-2,4a,5,8a-tetramethyl-1-naphthyl formate

Cas Number:

65405-72-3

Molecular formula:

C15H24O2

IUPAC Name:

1,2,3,4,4a,7,8,8a-octahydro-2,4a,5,8a-tetramethyl-1-naphthyl formate

Test material form:

liquid

Test animals

Species:

guinea pig

Strain:

not specified

Details on test animals or test system and environmental conditions:

No data

Test system

Type of coverage:

open

Preparation of test site:

clipped

Vehicle:

other: Acetone

Controls:

no

Amount / concentration applied:

0.025 mL at concentrations of 3, 10, 30 and 100 %

Duration of treatment / exposure:

24 hours

Observation period:

24 hours

Number of animals:

No data

Details on study design:

A single application of 0.025 mL of each test concentration was simultaneously performed on an area measuring 2 cm2 of the flank of skin previously clipped and marked with a circular stamp.
The skin reactions were read 24 hours after application of the test material. The minimum and maximum non-irritant concentrations were determined by an all-or-none criterion. The minimal irritant concentration was defined as the lowest one causing skin irritation. The maximal non-irritant concentration was defined as the highest one not causing macroscopic skin irritation in any of the animals.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

other: Lowest irritant concentration

Basis:

other: No data

Time point:

other: 24 h

Score:

10

Reversibility:

no data

Results 2

Irritation parameter:

other: Highest non-irritant concentration

Basis:

other: No data

Time point:

other: 24 h

Score:

3

Reversibility:

no data

Any other information on results incl. tables

Skin irritation after a single application

Solvent	Lowest irritant concentration	Highest non-irritant concentration
Acetone	10 %	3 %

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

After a single application of the test substance, a 3% concentration in acetone does not cause skin irritation. Application of 10 % in acetone caused irritation.
After 7 days continuous application of the test item, only mild irritation was observed.

Executive summary:

The skin irritation potential of the test substance was assessed in guinea pigs.

After a single application of the test substance a 3% concentration in acetone did not irritate the skin.

A 10 % concentration was the lowest irritant concentration after a single application of the test substance.

This non-classification is based on the additional information after 21 days repeated dosing at 3%, 10%, 30% and 100% where after 7 days dosing at 100%, only slight irritation was observed.

Therefore after a single dose, it is extremely unlikely that oxyoctaline formate would be an irritant after 7 days and therefore would not be classifiable.