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EC number: 229-043-5 | CAS number: 6407-78-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary source.
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Draft Screening Assessment: Aromatic Azo and Benzidine-based Substance Grouping - Certain Azo Solvent Dyes
- Author:
- Environment Canada - Health Canada; November 2013
- Year:
- 2 013
- Bibliographic source:
- Draft Screening Assessment Certain Azo Solvent Dyes 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of Solvent Yellow 18 in Salmonella Typhimurium TA98, TA100 by modified AMES test.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-[(2,4-dimethylphenyl)azo]-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-one
- EC Number:
- 229-043-5
- EC Name:
- 4-[(2,4-dimethylphenyl)azo]-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-one
- Cas Number:
- 6407-78-9
- Molecular formula:
- C18H18N4O
- IUPAC Name:
- 4-[(2,4-dimethylphenyl)azo]-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-one
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (IUPAC name): 4-[(E)-2-(2,4-dimethylphenyl)diazen-1-yl]-3-methyl-1-phenyl-4,5-dihydro-1H-pyrazol-5-one
- Common name: Solvent Yellow 18
- Molecular formula: C18H18N4O
- Molecular weight: 306.367 g/mol
- Smiles notation: O=C1N(N=C(C)[C@@H]1\N=N\c1c(cc(cc1)C)C)c1ccccc1
- InChl: 1S/C18H18N4O/c1-12-9-10-16(13(2)11-12)19-20-17-14(3)21-22(18(17)23)15-7-5-4-6-8-15/h4-11,17H,1-3H3/b20-19+
- Substance type: Organic
- Physical state: Solid
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 metabolic activation containing flavin-mononucleotide (FMN)
- Test concentrations with justification for top dose:
- Not specified
- Vehicle / solvent:
- Not specified
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- Method of application: Pre incubation
- Rationale for test conditions:
- Not specified
- Evaluation criteria:
- Not specified
- Statistics:
- Not specified
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- Not specified
- Remarks on result:
- other: No mutagenic effect were observed
Applicant's summary and conclusion
- Conclusions:
- The mutagenicity of Solvent Yellow 18 was tested in modifying Ames test in S. typhimurium strainsTA98 and TA100 under standard and reductive (with FMN) conditions and with metabolic activation. Positive results were observed in strain TA98, regardless of the presence of FMN, and the results were negative in strain TA100, with and without FMN
- Executive summary:
The mutagenicity of Solvent Yellow 18 was tested in modifying Ames test in S. typhimurium strains TA98 and TA100 under standard and reductive (with FMN) conditions and with metabolic activation. Positive results were observed in strain TA98, regardless of the presence of FMN, and the results were negative in strain TA100, with and without FMN. Therefore Solvent Yellow 18 result for this test was considered to be ambiguous.
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