2-phenoxyethyl methacrylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The experimental study was performed according to DIN 38 412, which is comparable to OECD Guideline. However the loading of the test species is 4 times higher than suggested maximum according to the OECD guideline 203. In additon the study is not GLP.

Qualifier:

according to guideline

Guideline:

other: DIN 38 412, L1 and L15

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

The species tested is not recommended in the guideline

GLP compliance:

not specified

Analytical monitoring:

no

Details on sampling:

The mortality and behavior of test species were evaluated at times, 1,4,24,48,72 and 96 hours.

Details on test solutions:

The concentration range was selected on the basis of a range finding study, were a LC50 of 10 mg/l were indicated.
the concentration range was 1.0-21.5 mg/l, spaced by a factor of 2.15

Test organisms (species):

Leuciscus idus

Details on test organisms:

The ide, Leuciscus idus is a widely distributed freshwater cyprinid. Body length was 6.9-8.9 cm and weigth 2.8-6.6 gram.
Fish were kept in a flow-through tank in tap water cleaned active carbon and aerated with oil-free air.
Photoperiod, 16:8, light : dark
Total hardness about 2.5 mmol/l
Acid capacity about 5.5 mmol/l
Oxygen content > 60% of maximum saturation
pH: about 8.0
Duration of housing and adaptation, about 4 months
Water temperature 13-22 C
Mortality during the last two weeks of housing about 0.1%
Mortality during adaptation period 0%
Medical treatment; 4 times with 0.05mg/l malachite green chloride
2 times with 10 mg/l tetracycline hydrochloride.
Diet: Growing Feed F/B 50, obtained from SSNIFF Spezialdiaeten GMBH D-4770 Soest, FRG, ad libitum.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

4 months

Hardness:

2.5 mmol/l

Test temperature:

13-22 oC

pH:

about 8.0

Dissolved oxygen:

7.8-8.5 mg/l

Salinity:

freshwater

Nominal and measured concentrations:

Nominal concentrations; 1.0, 2.15, 4.64, 10.0, and 21.5 mg/l

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 10 - <= 22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

The positive control was performed with chloroacetamide. The LC50 after 48 hours was about 26 mg/l, which correspond to the normal sensitivity of the test species. The experimental setup is therefore considered valid.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 values of phenoxyethyl acetate to the ide, Leuciscus idus, was established to about 11.5 mg/l (48h) and 10.0 mg/l (72h and 96h).

Executive summary:

The short-term toxicity of phenoxyethyl acrylate to the fish ide, Leuciscus idus, was tested according to DIN 38 412 (L1 and L15). The method is similar to OECD Guideline 203, except that the species used is not directly recommended by the OECD Guideline. The fish is however considered relevant, as it is a widespread European freshwater fish of the cyprinid family.

The LC50 values were established to about 11.5 mg/l (48h) and 10.0 mg/l (72h and 96h).

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Read-across from short-term toxicity study on one closely structural and physicochemical related substance: 2-Phenoxyethyl acrylate (M140).
For further read-across justification se document attached in section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 10 - <= 22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Executive summary:

No data is available for 2-Phenoxyethyl methacrylate (T).

The short-term toxicity of 2-phenoxyethyl acrylate to the fish, Leuciscus idus, was tested according to DIN 38 412 (L1 and L15). The is method similar to OECD test Guideline 203 except that the species used is not directly recommended by the OECD Guideline. The fish is however considered relevant, as it is a widespread European freshwater fish of the cyprinid family. The LC50 values were established to about 11.5 mg/l (48h) and 10.0 mg/l (72h and 96h).

Due to structural and physico-chemical similarity to 2-Phenoxyethyl methacrylate, a 48 h LC50 about 11.5 mg/l, and a 72 h and 96h LC50 of 10.0 mg/l can also be concluded for 2-Phenoxyethyl methacrylate (T) as well.

See justification for read-across attached in section 13.

Description of key information

Based on a read-across to the measured toxicity to fish of the source substance, LC50 to fish is derived at:

LC50 (Leuciscus idus, 96hr) = 10 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10 mg/L

Additional information

The short-term toxicity of phenoxyethyl acrylate (S) to the fish ide, Leuciscus idus, was tested according to DIN 38 412 (L1 and L15). The method is similar to OECD Guideline 203, except that the species used is not directly recommended by the OECD Guideline. The fish is however considered relevant, as it is a widespread European freshwater fish of the cyprinid family.

The LC50 values were established to about 11.5 mg/l (48h) and 10.0 mg/l (72h and 96h).