2-(diphenylphosphino)benzonitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-guideline test. No GLP. Poorly written report. No pathology.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

not specified

Principles of method if other than guideline:

On day 0 of doisng, rats were starved for 4 hours, then weighed before dose adminstration by gavage.
Each dose was kept constant by varying the volume dispensed according to individual body weights.
Following dosing, food and water were witheld for one hour.
Animals were observed for signs of toxicity at 0.5, 1,2, 4 and 6 hours after dosing. Body weights were recorded on day 0, 7 and 14.
All surviving animals were destroyed on day 14.

GLP compliance:

not specified

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(diphenylphosphanyl)benzonitrile

Cas Number:

34825-99-5

Molecular formula:

C19H14NP

IUPAC Name:

2-(diphenylphosphanyl)benzonitrile

Test material form:

solid - liquid: suspension

Details on test material:

The solid substance was made up as a 100% w/v suspension in corn oil.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

Bought from commercial supplier (Bantam & Kingman Ltd).
Body weight range 200-225 g.
On arrival, animals were housed in single sex groups of up to 5 per cage.
Cages were mounted in 5 rows of 4 cages stacked.
Food and water provide ad libitum.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

Only acclimatised male rats used.
On Day 0 - dosing - animals were starved for 4 hours and weighed.
Doses were administered by gavage using a mounted syringe, which allowed for accurate dispension of 2000 mg/kg.
After dosing, food and water were withheld for 1 hour.

Doses:

The administered dose was 2000 mg/kg bw per animal.
This amounted to a syringable volume of between 2 and 5 cm3 per kg body weight.

No. of animals per sex per dose:

2 Males

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

None reported.

Clinical signs:

other: None reported.

Gross pathology:

None reported.

Other findings:

None reported.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The oral LD50 of 2-(diphenylphospihino)-benzonitrile is greater than 2000 mg/kg bw.
No overt signs of toxicity were seen. No treatment related signs or body weight decreases were observed.