Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from authorative database

Data source

Reference
Reference Type:
other: authorative database
Title:
Hazardous Substances Data Bank (HSDB) for 2-METHYL-1,4-BENZENEDIAMINE
Author:
U.S National Library of Medicine
Year:
2017
Bibliographic source:
Hazardous Substances Data Bank (HSDB) for 2-METHYL-1,4-BENZENEDIAMINE U.S National Library of Medicine 2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To evaluate eye irritation potential of 2,5-Di amino toluene on rabbit’s eye
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methyl-p-phenylenediamine
EC Number:
202-442-1
EC Name:
2-methyl-p-phenylenediamine
Cas Number:
95-70-5
Molecular formula:
C7H10N2
IUPAC Name:
2-methylbenzene-1,4-diamine
Test material form:
solid
Details on test material:
-IUPAC name:2-methylbenzene-1,4-diamine
-Molecular formula :C7H10N2
-Molecular weight :122.17 g/mol
- Substance type:organic
- Physical state: solid
-InChI:1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
-Smiles:Cc1cc(ccc1N)N

Specific details on test material used for the study:
-IUPAC name:2-methylbenzene-1,4-diamine
-Molecular formula :C7H10N2
-Molecular weight :122.17 g/mol
- Substance type:organic
- Physical state: solid
-InChI:1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
-Smiles:Cc1cc(ccc1N)N

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
other: aqueous sodium sulphite (pH 7.0)
Amount / concentration applied:
0.1 mL of a 2.5% w/v
Duration of treatment / exposure:
10 seconds
Observation period (in vivo):
1 hr , Days 1, 2, 3, 4, and 7.
Number of animals or in vitro replicates:
Total=3
Details on study design:
Washing (if done): eye was rinsed with 50 mL of lukewarm water
SCORING SYSTEM: Draize scoring system

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
7 d
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Mild conjunctival irritation was observed in 2 animals on days 1 and 3 respectively

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
2,5-Diaminotoluene was installed into the eye of three rabbits for in the concentration 0.1 mL of a 2.5% w/v in aqueous sodium sulphite (pH 7.0) for 10 sec. Mild conjunctival irritation was observed in 2 animals on days 1 and 3 respectively. Hence, 2,5-Diaminotoluene was considered to be slightly irritating to rabbit eye.
Executive summary:

2,5-Diaminotoluene was used as a test material to evaluate ocular irritation potential on three rabbits. The test material was installed onto the eyes of rabbits in the concentration 0.1 mL of a 2.5% w/v in aqueous sodium sulphite (pH 7.0) for 10 sec. After 10 seconds the eye was rinsed with 50 mL of lukewarm water.eyes were evaluated and scored by Draize scoring system at 1 hr and then at Days 1, 2, 3, 4, and 7.

Mild conjuctival irritation was observed in 2 animals on day 1 and 3 respectively . No data regarding reversibility of the irritation was stated. Under the conditions of this test, toluene-2,5-diamine solution caused slight irritation to rabbit eyes.