Registration Dossier

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 21, 2011 to October 04, 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
yes
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(Z)-hex-3-enyl isobutyrate
EC Number:
255-424-0
EC Name:
(Z)-hex-3-enyl isobutyrate
Cas Number:
41519-23-7
Molecular formula:
C10H18O2
IUPAC Name:
(Z)-hex-3-enyl isobutyrate
Specific details on test material used for the study:
TEST MATERIAL
Name ( stated on the report) : Hexenyl-3-cis Isobutyrate
Appearance: Colourless to pale yellow liquid
Batch: VE00153050
Expiry date: April 22, 2012

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
other: Sodium benzoate (Fluka, Buchs, Switzerland, Art. No. 71300), purity : min. 99.0 %

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
78
Sampling time:
28 d
Details on results:
The 10-day window criterion is also fulfilled (19 % biodegradation on day 2 and 64 % on day 12).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Hexenyl-3-cis Isobutyrate undergoes 78 % biodegradation after 28 days in the test conditions.
The 10-day window criterion is also fulfilled (19 % biodegradation on day 2 and 64 % on day 12).
Hexenyl-3-cis Isobutyrate did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Thus, Hexenyl-3-cis Isobutyrate should be regarded as readily biodegradable according to this test.
Executive summary:

The Ready Biodegradability of Hexenyl-3-cis Isobutyrate was determined by the Manometric Respirometry Test.

Hexenyl-3-cis Isobutyrate undergoes 78 % biodegradation after 28 days in the test conditions.

The 10-day window criterion is also fulfilled (19 % biodegradation on day 2 and 64 % on day 12).

Hexenyl-3-cis Isobutyrate did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

Thus, Hexenyl-3-cis Isobutyrate should be regarded as readily biodegradableaccording to this test.