Zirconium carbide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 17-21, 2011

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

17th July 1992

Deviations:

no

GLP compliance:

not specified

Remarks:

The report does not contain a statement on GLP. In regard to animal welfare it is refrained to repeat this acute toxicity test in GLP compliance. Especially, because data for acute fish toxicity is not demanded in annex VII of the REACH regulation.

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 20031/11
- Expiration date of the lot/batch: July 31, 2012
- Purity: 99.5 %

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

no

Details on test solutions:

Proper weights of the test substance (1.0004 g-1.0009 g) were added into the jar filled with 10 L water. Before the fish were exposed the test solutions were stirred with magnetic force with the speed of 450 rpm for 1 hour. After 1 hour of stirring the speed of the magnetic force rotor was maintained at 200 rpm and then 5 fish was put into the test vessel.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish (Danio rerio)
- Strain: /
- Length at study initiation (length definition, mean, range and SD): 2.601 ± 0.234cm
- Weight at study initiation (mean and range, SD): 0.2718 ± 0.071 g

ACCLIMATION
- Acclimation period: at least 12 days

Feeding: none

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

22.2 - 22.9 °C

pH:

7.62 - 7.96

Dissolved oxygen:

6.40 - 9.07 mg/L

Nominal and measured concentrations:

0, 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass jars
- Type (delete if not applicable): open
- Material, size, fill volume: diameter: glass; 21cm, high: 31 cm, 12cmx 6cm; 10 L
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): daily; reconstituted water after aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Loading: < 1.0 g fish/L water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12:12
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

During the 96-hours exposure period, no mortality and toxic symptoms were found in control group and treatment group.

Validity criteria fulfilled:

yes

Conclusions:

No toxic effects in Danio rerio could be observed after exposure to 100 mg/L ZrC (semi-static exposure) after 96 h.

Executive summary:

A study was performed to assess the acute toxicity to the zebrafish (Danio rerio) of ZrC. A preliminary test was conducted using one test concentration of 100 mg/L. A concurrent water control group was performed in parallel. 5 fish were used in each concentration. The preliminary test was performed with a semi-static water renewal system. Dissolved oxygen and pH values were determined at the beginning of the test and at 24-hour intervals during the exposure period.

During the 96-hours exposure period. No mortality and toxic symptoms were found in control group and treatment group.
Based on the results, the 96 h LC₅₀ of ZrC was greater than 100 mg/L (nominal concentration) to fish under the experiment condition of this study.

Description of key information

A study was performed to assess the acute toxicity to the zebrafish (Danio rerio) of ZrC. A preliminary test was conducted using one test concentration of 100 mg/L. A concurrent water control group was performed in parallel. 5 fish were used in each concentration. The preliminary test was perfromed with a semi-static water renewal system. Dissolved oxygen and pH values were determined at the beginning of the test and at 24-hour intervals during the exposure period.

During the 96-hours exposure period. No mortality and toxic symptoms were found in control group and treatment group.
Based on the results, the 96 h LC₅₀of ZrC was greater than 100 mg/L (nominal concentration) to fish under the experiment condition of this study.