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EC number: 701-083-2 | CAS number: -
ThCO2, expressed as mg CO2/mg test substance, was calculated from the results of carbon analysis. The first step in calculating the amount of CO2 produced is to correct for background (endogenous) CO2 production. Thus the amount of CO2 produced by a test substance is determined by the difference (in ml of titrant) between the experimental and blank Ba(OH)2 traps. The amount of 0.05 N HCl titrated is converted into mg of CO2 produced: 44 1.1 ml HCl titrated 2 0.05 ml HCl titrated mg CO2 Relative biodegradation values were calculated from the cumulative CO2 production relative to the total expected CO2 production based on the total carbon content of the amount of test substance present in the test bottles. A figure of more than 10% biodegradation was considered significant. The relative biodegradation values were plotted versus time together with the relative biodegradation of the positive control. If applicable, the number of days is calculated from the attainment of 10% biodegradation until 60% biodegradation. Should this period be ≤ 10 days (10-day window), then the test substance is designated as readily biodegradable. Toxicity control: if less than 25% biodegradation (based on ThCO2) occurred within 14 days, the test substance was assumed to be inhibitory. The total CO2 evolution in the inoculum blank was determined by the cumulative difference (in ml of titrant) between the blank Ba(OH)2 traps and fresh Ba(OH)2.
Determination of ‘ready’ biodegradability: carbon dioxide (CO2) evolution test (modified Sturm test) with MEA Polyborate 1:1.
The study procedures described in this report were based on the OECD guideline No. 301 B, 1992. In addition, the procedures were designed to meet the test methods of theCommissionRegulation (EC) No. 440/2008 of 30 May 2008, Publication No.L142, Part C.4-C and the ISO International Standard 9439, 1999.
The batch of MEA Polyborate 1:1 tested was aclear colourless liquid.MEA Polyborate 1:1 was tested as delivered by the sponsor. The organic carbon content ofMEA Polyborate 1:1could not be calculated and therefore the Total Organic Carbon (TOC) content was determined.
The TOC content ofMEA Polyborate 1:1was determined to be 14.42%.MEA Polyborate 1:1was tested in duplicate at 136.5 mg/l, corresponding to 20 mg TOC/l.Based on the TOC content the ThCO2of MEA Polyborate 1:1 was calculated to be 0.53 mg CO2/mg.
The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles (MEA Polyborate 1:1),
- 1 positive control (sodium acetate) and
- 1 toxicity control (MEA Polyborate 1:1 plus sodium acetate).
Weighed amounts of MEA Polyborate 1:1 were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. To this end, 20 ml of Milli-RO water was added to each weighing bottle containing the test substance. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms. Test duration was 28 days (last CO2-measurement on the 29thday).
The relative biodegradation values calculated from the measurements performed during the test period revealed 69 and 77% biodegradation of MEA Polyborate 1:1 for A and B, respectively.
Furthermore, in test bottle B biodegradation of MEA Polyborate 1:1 of at least 60% was reached within a 10-day window.
In the toxicity control, MEA Polyborate 1:1 was found not to inhibit microbial activity.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, MEA Polyborate 1:1 is designated as readily biodegradable.
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