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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
Version / remarks:
Adopted: July 29, 2016
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

In vitro test system

Test system:
human skin model
Source species:
human
Vehicle:
unchanged (no vehicle)
Control samples:
yes, concurrent no treatment
Amount/concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50µl

NEGATIVE CONTROL
- Amount(s) applied (volume or weight): 50 µl water

POSITIVE CONTROL
- Amount(s) applied (volume or weight): 50 µl
Duration of treatment / exposure:
3-minute and 60-minute exposures were conducted
Number of replicates:
duplicates

Results and discussion

In vitro

Resultsopen allclose all
Irritation / corrosion parameter:
% tissue viability
Remarks:
3-minute exposure
Run / experiment:
1
Value:
14.9
Negative controls valid:
yes
Positive controls valid:
yes
Irritation / corrosion parameter:
% tissue viability
Remarks:
3-minute exposure
Run / experiment:
2
Value:
23.8
Negative controls valid:
yes
Positive controls valid:
yes
Irritation / corrosion parameter:
% tissue viability
Remarks:
3-minute exposure
Run / experiment:
mean
Value:
19.4
Negative controls valid:
yes
Positive controls valid:
yes
Irritation / corrosion parameter:
% tissue viability
Remarks:
60-minutes exposure
Run / experiment:
1
Value:
7.7
Negative controls valid:
yes
Positive controls valid:
yes
Irritation / corrosion parameter:
% tissue viability
Remarks:
60-minutes exposure
Run / experiment:
2
Value:
9.2
Negative controls valid:
yes
Positive controls valid:
yes
Irritation / corrosion parameter:
% tissue viability
Remarks:
60-minutes exposure
Run / experiment:
mean
Value:
8.5
Negative controls valid:
yes
Positive controls valid:
yes

Applicant's summary and conclusion

Interpretation of results:
Category 1A (corrosive) based on GHS criteria
Conclusions:
The ability of 1-Diethylaminopropan-2-ol to induce skin corrosion was investigated by using EpiDerm™SCT (EPI-200).
As a result of the skin corrosion test in the 3-minute and 60-minute exposures, the tissue viabilities treated by the test substance were 19.4% and 8.5%, respectively.
Consequently, it was concluded that 1-Diethylaminopropan-2-ol is "Corrosive" (UN GHS Category 1A) under the present test conditions.