3,4-dicyanoaniline

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from peer reviewed journal.

Data source

Reference

Reference Type:

publication

Title:

Final amended report of the safety assessment of toluene-2,5-diamine, toluene-2,5-diamine sulfate, and toluene-3,4-diamine as used in cosmetics

Author:

Cosmetic Ingredient Review Expert Panel

Year:

2010

Bibliographic source:

International Journal of Toxicology; 2010 May;29(3 Suppl):61S-83S

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

To evaluate eye irritation potential of 2,5-Diaminotoluene (CAS no: 95-70-5) on rabbit’s eye.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-methyl-p-phenylenediamine

EC Number:

202-442-1

EC Name:

2-methyl-p-phenylenediamine

Cas Number:

95-70-5

Molecular formula:

C7H10N2

IUPAC Name:

2-methylbenzene-1,4-diamine

Test material form:

solid

Details on test material:

Name: 2-methyl-p-phenylenediamine
InChI: 1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
Smiles: Cc1cc(ccc1N)N
- Molecular formula :C7H10N2
- Molecular weight :122.17 g/mol
- Substance type:Organic

Specific details on test material used for the study:

Name: 2-methyl-p-phenylenediamine
InChI: 1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
Smiles: Cc1cc(ccc1N)N
- Molecular formula :C7H10N2
- Molecular weight :122.17 g/mol
- Substance type:Organic

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

Not specified

Test system

Vehicle:

other: 0.05 %aqueous sodium sulphite (0.1mL pH 7.0)

Controls:

not specified

Amount / concentration applied:

2.5% (0.1mL)

Duration of treatment / exposure:

10 seconds

Observation period (in vivo):

1 hr, Days 1, 2, 3, 4, and 7.

Number of animals or in vitro replicates:

3

Details on study design:

Washing (if done): eye was rinsed with 50 mL of lukewarm water
SCORING SYSTEM: Draize scoring system

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

7 d

Reversibility:

not fully reversible within:

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No ocular lesions were observed in treated rabbits.

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating

Conclusions:

No ocular lesions were observed in treated rabbits. Hence the chemical 2,5-Diaminotoluene (CAS no: 95-70-5) was considered to be not irritating to the rabbits’ eye.

Executive summary:

An eye irritation study was conducted to assess its eye irritation potential of chemical2,5-Diaminotoluene (CAS no:95-70-5).

 

The test material was installed onto the eyes of rabbits in the concentration 0.1 mL of a 2.5% w/v in aqueous sodium sulphite (pH 7.0) for 10 sec. After 10 seconds the eye was rinsed with 50 mL of lukewarm water. The treatedeyes were evaluated and scored by Draize scoring system at 1 hr and then at Days 1, 2, 3, 4, and 7.

 

Since none of the treated rabbit produced any ocular lesions, the chemical2,5-Diaminotoluene (CAS no:95-70-5) was considered to be not irritating to the rabbits’ eye.