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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Basic toxicokinetics

Currently viewing:

Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Study period:
Prior to or equal to 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Unknown whether GLP study. No Guideline available. Sufficient data available for interpretation of results.
Justification for type of information:
Read across is based on the category approach. Please refer to attached category document.

Data source

Reference
Reference Type:
publication
Title:
Oxidation of polyethylene glycols by alcohol dehydrogenase
Author:
Herold, DA, Keil, KA, and Bruns, DE.
Year:
1989
Bibliographic source:
Biochemical Pharmacology 38, 73-76.

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
no guideline available
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3,6,9-trioxaundecane-1,11-diol
EC Number:
203-989-9
EC Name:
3,6,9-trioxaundecane-1,11-diol
Cas Number:
112-60-7
Molecular formula:
C8H18O5
IUPAC Name:
2,2'-[oxybis(ethane-2,1-diyloxy)]diethanol
Radiolabelling:
no

Test animals

Species:
other: horse
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
other: in vitro assay
Vehicle:
unchanged (no vehicle)
Duration and frequency of treatment / exposure:
No data.
Doses / concentrations
Remarks:
Doses / Concentrations:
No data.
No. of animals per sex per dose / concentration:
Not applicable.
Control animals:
other: in vitro assay run without alkaline phosphatase and the inhibitor of alkaline phosphatase, 4-methylpyrazole

Results and discussion

Preliminary studies:
Not applicable

Toxicokinetic / pharmacokinetic studies

Details on absorption:
Not applicable
Details on distribution in tissues:
Not applicable
Details on excretion:
Not applicable

Metabolite characterisation studies

Metabolites identified:
no
Details on metabolites:
There was no appreciable reaction in the absence of alcohol dehydrogenase. Reaction was completely inhibited by 4-methylpyrazole. Vmax and Km values are tabulated below.

Bioaccessibility (or Bioavailability)

Bioaccessibility (or Bioavailability) testing results:
Not applicable.

Any other information on results incl. tables

Table 1 Vmax and Km values in in vitro equine alkaline phosphatase studies

 Substrate  Km (mM)  Vmax (nmol/min)
 EG

 1000 ± 60

 64 ± 1

 DEG

 1900 ± 300

 27 ± 5

 TEG

 810 ± 50

 19 ± 2

 TetraEG

 490 ± 20

 17 ±1
 PentaEG

 340 ± 20

 12 ± 2

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): bioaccumulation potential cannot be judged based on study results
The ethylene glycols are in vitro substrates for alcohol dehydrogenase. Vmax for metabolism of the ethylene glycols consistently decreases with the number of glycol units. Km consistently decreased with the number of glycol units with the exception of DEG
Executive summary:

The ability of equine alkaline phosphatase to degrade various ethylene glycol compounds was examined in vitro.

The ethylene glycols are in vitro substrates for alcohol dehydrogenase. Vmax for metabolism of the ethylene glycols consistently decreases with the number of glycol units. Km consistently decreased with the number of glycol units with the exception of DEG