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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
30 May - 01 Jun 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
adopted in 1982
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution contained 100 mg/L test item and 100 mg/L Tween 80 and was stirred for 60 min at 293 K. A serial dilution with dilution water was prepared and each test vessel received 10 mL of the respective dilution.
- Chemical name of vehicle: Tween 80
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Laboratory breed at BASF, originally obtained in 1978 from the Institut National de Recherche Chimique Appliquée, France
- Age at study initiation (mean and range, SD): 2 - 24 h

ANIMAL HUSBANDRY
- Light: 16 h light/8 h dark, 5 µE/m2 x s, 400 - 750 nm
- Type and amount of food: Green algae
- Feeding frequency: Once daily

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.77 mmol/L
Test temperature:
292.5 - 293.8 K
pH:
7.77 - 8.14
Dissolved oxygen:
8.36 - 8.87
Nominal and measured concentrations:
Nominal: 0, 0.098, 0.195, 0.39, 0.781, 1.56, 3.12, 6.25, 12.5, 25.0, 50.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Centrifuge glass vessels with flat bottom
- Material, fill volume: Glass, 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water
- Ca/Mg ratio: 4:1
- Conductivity: 600 - 700 µS/cm
- Intervals of water quality measurement: Temperature, oxygen and pH were measured at test start and test end.

OTHER TEST CONDITIONS
- Light intensity: Diffuse light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Immobilisation was recorded after 0, 3, 6, 24 and 48 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.53 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.47 - 0.59
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.39 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
0.781 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: No mortality
- Immobilisation: 5% immobilisation at 0.39 mg/L, 100% immobilisation at 0.781 to 50 mg/L
Reported statistics and error estimates:
Determination of EC50 (48h) by moving average method

Table 1: Immobilisation of Daphnia magna

Concentration (mg/L)

Mobile Daphnia after

0 h

24 h

48 h

0

20

20

20

0.098

20

20

20

0.195

20

20

20

0.39

20

20

19

0.781

20

7

0

1.56

20

0

0

3.12

20

0

0

6.25

20

0

0

12.5

20

0

0

25.0

20

0

0

50.0

20

0

0

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21 Jul - 24 Jul 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted in 2004
Deviations:
yes
Remarks:
Higher temperature during the test (Guideline: 18 - 22 °C, Test: 23.5 - 23.7 °C)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
adopted in 2008
GLP compliance:
yes (incl. certificate)
Remarks:
Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
Analytical monitoring:
yes
Details on sampling:
- Concentrations: All concentrations were measured at test start and test end.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution containing 10 g/L was prepared using the following procedure: 10 g test item was mixed with 1.1 g NaOH (45%). 40 mL deionised water (80 – 100 °C) was added. After homogenisation with a glass stirrer, 85 mL deionised water (room temperature) was added. Then, the vessel was stirred on a magnetic stirrer. 0.5 M NaOH was added until the resulting solution was clear. The pH of the solution was adjusted to 7.8 ± 0.2 with 1 M HCl. the solution was transferred into a volumetric flask and filled up to 1000 mL with deionised water. From this solution, the concentrations to be tested were prepared by diluting with dilution water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Umweltbundesamt Berlin, Germany, received on 27 Sep 2007
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: None

ANIMAL HUSBANDRY
- Method of breeding: Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept in 2L glass beakers in M4 medium and multiply by parthenogenesis thus being genetically identical.
- Type and amount of food: Unicellular green algae
- Feeding frequency: Twice a week
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
250 mg CaCO3/L
Test temperature:
23.2 - 23.7 °C
pH:
7.3 - 7.8
Dissolved oxygen:
6.4 - 8.3 mg/L
Nominal and measured concentrations:
Nominal: 0, 0.1, 0.22, 0.46, 1.0, 2.2, 4.6, 10, 22, 46, 100 mg/L
Mean measured: < LOQ, < LOQ, < LOQ, < LOQ, 0.42, 0.60, 1.77, 5.58, 12.14, 28.94, 64.70 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Beakers
- Material, size, fill volume: Glass, 50 mL, 20 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water was used for the preparation of M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen concentration were measured at test start and test end.

OTHER TEST CONDITIONS
- Photoperiod: Darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- After 24 and 48h the immobilised daphnia were counted.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.43 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.38 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: 5% after 48 h
- Immobilisation: 10% at nominal 0.46 mg/L, 70% at nominal 1.0 mg/L, 100% at nominal 2.2 mg/L to 100 mg/L
- Analytical measurements: In treatments 0.1 – 0.46 mg/L (nominal) no test item was detectable after 48 h. The recovery after 48 h in treatments 1 – 100 mg/L (nominal) was in the range of 44 – 128% of the start concentration. Because of the medium correlation between nominal and measured concentrations, the determination of the biological results was based on the geometric mean of the measured concentrations for treatments 1 – 100 mg/L (nominal) and on the measured concentration at test start for treatment 0.46 mg/L (nominal, measured: 0.38 mg/L). Treatments 0.1 and 0.22 mg/L were not used for the evaluation of the results.
Results with reference substance (positive control):
- The reference test was carried out 08 - 09 Jun 2010
- EC50 (24h): 1.6 mg/L, 95% CL = 1.4 - 1.9 mg/L
- NOEC (24h): 1.0 mg/L

Immobilities Exp. 1

Nominal Conc. In mg/L

Immobility 24 hours

Immobility 48 hours

abs.

in %

abs.

in %

0

0

0

0

0

0

0

0

0

0

0

1.0

3

3

4

2

60

4

4

4

2

70

2.2

5

5

5

5

100

5

5

5

5

100

4.6

5

5

5

5

100

5

5

5

5

100

10

5

5

5

5

100

5

5

5

5

100

22

5

5

5

5

100

5

5

5

5

100

46

5

5

5

5

100

5

5

5

5

100

100

5

5

5

5

100

5

5

5

5

100

 

Immobilities Exp. 2

Nominal Conc. In mg/L

Immobility 24 hours

Immobility 48 hours

abs.

in %

abs.

in %

0

1

0

0

0

5

1

0

0

0

5

0.1

0

0

0

0

0

0

0

0

0

0

0.22

0

0

0

0

0

0

0

0

0

0

0.46

0

0

0

0

0

1

1

0

0

10

 

Measured concentrations

Nominal Conc. in mg/L

Measured Conc. in mg/L

% Recovery after 48h

% of Nominal Conc.

Geom. Mean in mg/L

0 h

48 h

 

0 h

48 h

 

0

n.d.

n.d.

-

-

-

-

0.1

0.36

n.d.

0

363

--

--

0.22

0.37

n.d.

0

166

--

--

0.46

0.38

n.d.

0

82

--

--

1

0.51

0.35

70

51

35

0.42

2.2

0.90

0.40

44

41

18

0.60

4.6

2.20

1.43

65

48

31

1.77

10

5.80

5.36

93

58

54

5.58

22

12.65

11.64

92

58

53

12.14

46

27.02

30.99

115

59

67

28.94

100

57.21

73.17

128

57

73

64.70

 n.d. = not detectable

Validity criteria fulfilled:
yes

Description of key information

EC50 (48h) = 0.43 mg/L for Daphnia magna (OECD 202)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.43 mg/L

Additional information

Two studies investigating the short-term toxicity of N-methyl-N-(C18-(unsaturated)alkanoyl)glycine (EC 701-177-3) to aquatic invertebrates are available.

The key study was carried out according to OECD guideline 202 and GLP including analytical measurement. Daphnia magna was exposed to the following ten nominal concentrations 0.1, 0.22, 0.46, 1.0, 2.2, 4.6, 10, 22, 46, 100 mg/L under static conditions. These test concentrations were performed in two different tests. The first test was conducted using seven test concentrations (nominal: 1.0, 2.2, 4.6, 10, 22, 46, 100 mg/L). As immobilization (≥ 70%) was observed in all test concentrations, a second test was performed using three additional concentrations (nominal: 0.1, 0.22, 0.46 mg/L). Analytical measurements verified the test item concentration to be 44 – 128% of the initial test concentrations of 1 – 100 mg/L throughout the exposure period, 82 – 363% for the initial test concentrations of 0.1 – 0.46 mf/L. For the test concentrations 0.1 - 0.46 mg/L after 48 h test concentrations of < LOD are detectable. Immobility could be observed in concentrations ≥ 1 mg/L (nominal).The test revealed an EC50 (48h) of 0.43 mg/L (measured).

The supporting study was carried out under non-GLP conditions according to EU Method C.2 and without analytical measurement. Daphnia magna was exposed to ten test concentrations (0.098, 0.195, 0.39, 0.781, 1.56, 3.12, 6.25, 12.5, 25.0, 50.0 mg/L) under static conditions. Immobility of 100% could be observed in concentrations ≥ 0.781 mg/L. An EC50 (48h) of 0.53 mg/L (nominal) is reported.