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Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

4-chlororesorcinol was not mutagenic in an Amestest according to OECD guideline 471 in strains TA 1535, TA 1537, TA 98, TA 100 and TA 102 with and without metabolic activation.

In a mouse lymphoma assay in cell line L5178Y (tk+/--locus) according to OECD guideline 476,the test substance did not induce gene mutations with and without metabolic activation.

In an in vitro chromosome aberration test in Chinese hamster V79 cells according to OECD guideline 473,the test substance induced structural chromosome aberrations both in the presence and absence of metabolic activation.

Endpoint conclusion
Endpoint conclusion:
adverse effect observed (positive)

Genetic toxicity in vivo

Description of key information

4-chlororesorcinol was tested in an in vivo micronucleus study on NMRI mice according to OECD guideline 474.

Dose levels were selected according to toxicity observed in a pre-experiment.

In the main study,the analysis of blood samples of the males treated with the highest dose of 100 mg/kg bw. showed that the test item could be quantified in the blood confirming its bioavailability.

In addition,the mean number of polychromatic erythrocytes was slightly decreased after treatment with the test substance as compared to the mean value of PCEs of the vehicle control, indicating that 4-chlororesorcinol had some cytotoxic properties in the bone marrow.

Under the test conditions 3-amino-2-chloro-6-methylphenol was not clastogenic and/or aneugenic in the bone marrow cells of mice.

 

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (negative)

Additional information

Justification for classification or non-classification