3-isopropoxypropylamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19. Aug 1969 - 27. Aug 1969

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment. However, there is no analytical verification of the atmosphere concentrations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Principles of method if other than guideline:

Exposure of rats to an atmosphere saturated with vapours of isopropoxypropylamine generated at the temperature of 20 °C.

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Isopropoxypropylamine

IUPAC Name:

Isopropoxypropylamine

Constituent 2

Reference substance name:

3-isopropoxypropylamine

EC Number:

220-816-2

EC Name:

3-isopropoxypropylamine

Cas Number:

2906-12-9

Molecular formula:

C6H15NO

IUPAC Name:

3-(propan-2-yloxy)propan-1-amine

Details on test material:

- Name of test material (as cited in study report): Isopropoxy-propylamin
- Analytical purity: > 99 %

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 163 g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

no

Remarks on duration:

1 h, 3 h or 8 h

Concentrations:

1 h exposure: 48.5 mg/L
3 h exposure: 19.25 mg/L
8 h exposure: 18.5 mg/L
(nominal concentrations not verified by analytics).

No. of animals per sex per dose:

1 h exposure: 6
3 h and 8 h: 3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Frequency of weighing: day 0 and day 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC0

Effect level:

other: > saturated vapor atmosphere at 20°C

Exp. duration:

1 h

Remarks on result:

other: No mortality

Effect levels 2

Sex:

male/female

Dose descriptor:

LC50

Effect level:

other: > saturated vapor atmosphere at 20°C

Exp. duration:

3 h

Remarks on result:

other: mortality 1/6

Effect levels 3

Sex:

male/female

Dose descriptor:

LC50

Effect level:

other: < saturated vapor atmosphere at 20°C

Exp. duration:

8 h

Remarks on result:

other: mortality 5/6

Mortality:

3 h exposure: 1 animal died within 2 h of exposure.
8 h exposure: 3 animals died within 4.5 h of exposure and 2 further animals died within the end of exposure.

Clinical signs:

other: Initially escape attempts, irritation of the mucous membranes, dyspnoea. After the 3 and 8 h exposure also convulsions, staggering, corrosion of eyes and noses. 48 h post exposure in the 8 h exposure group intermittent respiration, respiratory sounds and

Body weight:

All surviving animals gained weight.

Gross pathology:

8 h exposure: 5x bloody nose crusts, lungs filled with blood, corroded snouts, sodden fur.

Any other information on results incl. tables

Mortality:

	24 h	7 days	14 days
1 h exposure	0 /12	0/12	-
3 h exposure	1/6	1/6	-
8 h exposure	5/6	5/6	5/6

Weight (g):

	day 0	day 7	day 14
1 h exposure	156	177	-
3 h exposure	176	227	-
8 h exposure	162	-	200

The inhalation of a highly enriched/saturated vapor-air-mixture may represent a hazard (mortality within 3 h). There is indication that the test substance causes local irritation to exposed tissues (including respiratory tract).

Applicant's summary and conclusion

Executive summary:

In a test to demonstrate the toxicity of an atmosphere saturated with vapours of the volatile components of isopropoxypropylamine at the temperature chosen for vapour generation (20 °C) (BASF, 1970), 3 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 1 h, 3 h or 8 h. The documentation of clinical signs was performed over a period of 7 days. During the 3 h exposure, 1/6 animal died within 2 h of exposure and for the 8 h exposure, 3/6 animals died within 4.5 h of exposure and 2/6 further animals died within the end of exposure. Escape attempts, irritation of the mucous membranes and dyspnoea were observed initially. After the 3 and 8 h exposure, convulsions, staggering, corrosion of eyes and noses were also noted. 48 h post exposure in the 8 h exposure group intermittent respiration, respiratory sounds and ruffled fur were noted. All surviving animals gained weight. For the 8 h exposure, bloody nose crusts, lungs filled with blood, corroded snouts, and sodden fur were observed in 5 rats.