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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, non-adapted
Details on inoculum:
- Origin: activated sludge from BASF's wastewater treatment plant
- Adaptation: none
Duration of test (contact time):
25 d
Initial conc.:
400 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Test performance:
- pH was neutralized before test initiation
- pH before neutralization: 9.7
pH after neutralization: 5.7 - 7.9
Parameter:
% degradation (DOC removal)
Value:
> 70
Sampling time:
25 d
Details on results:
- DOC elimination after 3 h (%): -1

- Degradation kinetics:
Time [d] Degradation [%]
1 -1
7 10
14 54
21 97
25 98
Interpretation of results:
inherently biodegradable
Conclusions:
The biodegradation of the test substance was >70% in a OECD 302 B Zahn-Wellens-Test. Thus, the test substance was inherently biodegradable.
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Deviations:
yes
Remarks:
COD analyzer was out of order for 12 days.The D0 and D4 samples were analyzed after 12 and 8 days storage in the fridge instead of 7 days allowed by the guideline. The results were still consistent. This deviation is considered to have no impact.
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Inoculum came from a secondary effluent from a municipal wastewater treatment plant (Versailles, 78, France). The sample was centrifuged at 4000 g for 20 minutes at 20°C. The pellet was dissolved in the test medium so that the solids were concentrated 150 times compared to the initial sample. The concentration of bacteria in the test medium was 2 900 000 bacteria per mL.
Duration of test (contact time):
28 d
Initial conc.:
244.5 mg/L
Based on:
act. ingr.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
The study followed the OECD 301A guideline. Eight flasks were used: two blanks, one control (sodium benzoate), two test item, one sterile, one for toxic inhibition and one for adsorption. Flasks were 500 mL capacity and filled with a volume of 250 mL. They were incubated at 22°C for 28 days. COD measurements occurred on days: 4, 7, 11, 14,18, 21, 25 and 28 (end of the study).
Before each sampling, water loss by evaporation were estimated by weighing the flasks. Water loss was compensated by adding quantity of ultra-pure water equivalent to the missing amount of water. Then, the flasks were shaken and sampled.
Reference substance:
benzoic acid, sodium salt
Preliminary study:
No preliminary study was conducted.
Key result
Parameter:
% degradation (DOC removal)
Value:
18
Sampling time:
28 d
Details on results:
3-isopropoxypropylamine biodegradation rate was low. Ultimate biodegradation reached only 18% after 28 days incubation. No significant adsorption was observed (<10%). The test item did not prove toxic to the inoculum. No abiotic ultimate biodegradation took place.
Results with reference substance:
Ultimate biodegradation of sodium benzoate was over 70% within 14 days.
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
3-isopropoxypropylamine is not readily biodegradable.
Executive summary:

The aim of this study was to evaluate ready biodegradability of 3 -isopropoxypropylamine. The study followed the OECD 301A guideline. Eight flasks were used: two blanks, one control (sodium benzoate), two test item, one sterile, one for toxic inhibition and one for adsorption. Flasks were 500 mL capacity and filled with a volume of 250 mL. They were incubated at 22°C for 28 days. COD measurements occurred on days: 4, 7, 11, 14,18, 21, 25 and 28 (end of the study). Before each sampling, water loss by evaporation were estimated by weighing the flasks. Water loss was compensated by adding quantity of ultra-pure water equivalent to the missing amount of water. Then, the flasks were shaken and sampled. The samples were centrifuged at 20°C. Supernatants were acidified with hydrochloric acid, degazed with nitrogen for 5 minutes and then analysed for COD with a dedicated analyzer. 3-isopropoxypropylamine biodegradation rate was low. Ultimate biodegradation reached only 18% after 28 days incubation. No significant adsorption was observed (<10%). The test item did not prove toxic to the inoculum. No abiotic ultimate biodegradation took place.

In conclusion, the test is valid and according to this test, 3-isopropoxypropylamine is not readily biodegradable.

Description of key information

The substance is not readily biodegradable according to an OECD 301A test. The substance is however inherently biodegradable according to an OECD 302 test.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information