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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2011-06-08 to 2011-08-01
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Batch: E011009938
Analytical monitoring:
Details on sampling:
0; 24, 48, 72, 96 h
Details on test solutions:
According to guideline
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): TWEEN 80 / SPAN 80 (3/1 v/v)
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)):

- Nominal values

Substance Concentration Solubilizer Tween 80 / SPAN-80
pos. Control 0.05 mg/L -
pos. Control 0.5 mg/L -
Test item: 33 mg/L 100 mg/L

Control: - 100 mg/L

Control - -

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Weight: 0.33 +/- 0.03 g
Length: 3.4 +/- 0.1 cm
Lot: 292-1
Date: 2011-06-08
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Post exposure observation period:
Test temperature:
25 +/- 0.1 °C
Nominal and measured concentrations:
33 mg/L
Details on test conditions:
10 fish per 4 L
Reference substance (positive control):
not specified
Key result
96 h
Dose descriptor:
Effect conc.:
> 33 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: with vehicle Tween 80 / Span 80 (3/1)
Sublethal observations / clinical signs:

- Mortality of control: 0 % after 96 hours

Mortality in %

 Sample              Mortality / [%]
 Test Material  Concentration / [mg/L]  24 h  48 h  72 h 96 h
 Positive Control  0.5  20  70  90  100
 Positive Control  0.05  0  0  0  0
 Test item (+vehicle)  33





 Tween 80 / SPAN-80 (3/1)












All concentrations tested were at or above the solubility limit of the pure test substance. To achive the exposure concentrations, solubiliser was used.

Validity criteria fulfilled:
The LC50 of the test material exceeds 33 mg/L and is clearly above the limit of water solubility.
Executive summary:

The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 203. The LC50 of the test material exceeds 33 mg/L and is clearly above the limit of water solubility.

Description of key information

GLP OECD 203 Assay: LC50 > 33 mg/L, NOEC = 33 mg/L (measured initial) with solubilizer

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
33 mg/L

Additional information

The LC50 of the test material exceeds 33 mg/L and is clearly above the limit of water solubility.