Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2013
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD 301 D non GLP
Principles of method if other than guideline:
- Principle of test: OECD 301 D
- Short description of test conditions: Ready Biodegradability study
- Parameters analysed / observed: Biodegradation of the substance during 28 days
GLP compliance:
no
Remarks:
Performed in US in 2013 but not for REACH purpose

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: flakes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: JHF-713-287
- Expiration date of the lot/batch: unknown
- Purity test date: 100%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
sewage, domestic
Details on inoculum:
Inoculum : effluent

Study design

Total exposure duration:
28 d

Test conditions

Test temperature:
20°C
pH:
pH at initiation from 7.44 to 7.57

Results and discussion

Effect concentrations
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
>= 2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: inhibition of activated sludge respiration
Details on results:
Bacterial plate counts at termination (Day 28) showed that the control, reference, and hydrogenated jojoba oil test substance treatments contained 2.2 × 104, 4.6 × 104, and 9.1 × 104 CFU/mL, respectively and indicated that the microbial inoculum remained consistent through the study and across the sample types. No inhibition was observed.

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Conclusions:
A NOEC (28 days) of 2 mg/L (nominal) can be reported for the inhibition of activated sludge respiration for Hydrogenated Jojoba Oil