Jojoba, ext., hydrogenated

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

no

Remarks:

Performed in April 2015 in US but not for REACH

Test material

Test material information

Constituent 1

Reference substance name:

Jojoba, ext., hydrogenated

EC Number:

296-292-4

EC Name:

Jojoba, ext., hydrogenated

Cas Number:

92457-12-0

Molecular formula:

No molecular formula is available since it is a UVCB substance

IUPAC Name:

Jojoba (Simmondsia Chinensis) Seed Oil Hydrogenated

Test material form:

solid: flakes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: JHF-713-287
- Expiration date of the lot/batch: unknown
- Purity test date: 100%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: stable in water, insoluble

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A growth inhibition test was performed from 18 to 21 March 2014 using a freshwater algal medium (FWAM) control and six test substance treatments at nominal concentrations of 0 (control), 0.30, 0.95, 3.1, 9.8, 31, and 100 mg hydrogenated jojoba oil/L. The test solutions were prepared as water accommodated fractions (WAF) by weighing 0.00030, 0.00095, 0.0031, 0.0098, 0.0310, and 0.10 g of jojoba wax flakes into individual 2.4-L glass jars containing 1 L of FWAM.
The control solution contained FWAM only.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Green Algae
- Strain: Pseudokirchneriella subcapitata
- Source (laboratory, culture collection): unknown
- Age of inoculum (at test initiation): unknown
- Method of cultivation:unknown

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

21.9 to 25.1 °C

pH:

pH at initiation : 7.3 - 7.4
pH at termination : 8.2 - 8.8

Nominal and measured concentrations:

Test concentrations : 0 (control), 0.30, 0.95, 3.1, 9.8, 31 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks, containing 100 mL of test solution
- Type (delete if not applicable): closed
- Aeration: The WAF solutions were stirred on an electric stir plate for 25 hours, with the stirring adjusted to provide a vortex <35% of the solution depth. The solutions were allowed to settle for approximately 1 hour before transferring into the test
chambers. Prior to transferring, approximately 100 mL of the solution was drained and discarded. All test chambers were labeled with the study number, treatment, replicate, and grid position.
- Initial cells density: 5000 cells/mL
- Control end cells density: 519 000 cells/mL
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 6 (control group), 3 (treatment groups)
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Photoperiod:Continuous

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Conclusions:

EC50 (growth rate, 72h) estimated to be > 100 mg/L
NOEC (72h) estimated to be > 100 mg/L
Not toxic