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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study (OECD 405). Non-GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
Method: according to Fed. Reg. 38 (187), §1500.42, p. 27029, 27-Sep-1973
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dimethoxyphenylacetonitrile
EC Number:
202-225-1
EC Name:
3,4-dimethoxyphenylacetonitrile
Cas Number:
93-17-4
Molecular formula:
C10H11NO2
IUPAC Name:
3,4-dimethoxyphenylacetonitrile
Test material form:
solid
Details on test material:
Veratrylcyanid, PSN 78/834; no data on purity given in the report.

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
- diet: SSNIFF, Intermast

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye
Amount / concentration applied:
undiluted
Amount applied: 30 mg (0.1 ml)
Duration of treatment / exposure:
24 h
Observation period (in vivo):
72 h
Number of animals or in vitro replicates:
6 (1 female and 5 males).

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal: #1-6
Time point:
other: 24 - 48 - 72 h
Score:
0
Max. score:
4
Irritation parameter:
iris score
Basis:
animal: #1-6
Time point:
other: 24 - 48 - 72 h
Score:
0
Max. score:
2
Irritation parameter:
conjunctivae score
Basis:
animal: #1-6
Time point:
other: 24 - 48 - 72 h
Score:
1.06
Max. score:
3
Reversibility:
other: A full reversibility of the symptoms within 21 days can be expected.
Remarks on result:
other: individual scores: 1.7, 1, 0.7, 1, 1, 1
Irritation parameter:
chemosis score
Basis:
animal: #1-6
Time point:
other: 24 - 48 - 72 h
Score:
0
Max. score:
4

Any other information on results incl. tables

Readings

Animal

Cornea

Iris

Conjunctiva

Additional findings

opacity

area

redness

chemosis

discharge

24 h

1

0

0

0

2

0

1

2

0

0

0

1

0

0

3

0

0

0

1

0

0

4

0

0

0

1

0

0

5

0

0

0

1

0

1

6

0

0

0

1

0

0

48 h

1

0

0

0

2

0

1

2

0

0

0

1

0

0

3

0

0

0

1

0

0

4

0

0

0

1

0

0

5

0

0

0

1

0

0

6

0

0

0

1

0

0

72 h

1

0

0

0

1

0

0

2

0

0

0

1

0

0

3

0

0

0

0

0

0

4

0

0

0

1

0

0

5

0

0

0

1

0

0

6

0

0

0

1

0

0

 Mean 24 -72h 1 0 0 1 .67   0  0.67  
  2 0 0  
  3 0 0 0.67   
  4 0 0  
  5 0 0 0.33   
  6 0 0  
 Mean 24 -72h 1 -6 0 0 0  1.06 0 0  0.17  

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information
Conclusions:
Based on the results of this study, the test substance vertarylcyanid does not need to be classified according to Regulation (EU) No. 1272/2008.