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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to acute tox. method. Non-GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
other: BASF Test
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dimethoxyphenylacetonitrile
EC Number:
202-225-1
EC Name:
3,4-dimethoxyphenylacetonitrile
Cas Number:
93-17-4
Molecular formula:
C10H11NO2
IUPAC Name:
3,4-dimethoxyphenylacetonitrile
Test material form:
solid
Details on test material:
Veratrylcyanid, PSN 78/834; no data on purity given in the report.

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female
Details on test animals or test system and environmental conditions:
- diet: Herilan MRH-Haltung; H. Eggersmann KG

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: 0.5 % aqueous CMC
Doses:
50, 200 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- way of application: intraperitoneal
- form of application: suspension
- formulation in: 0.5% aqueous carboxymethyl cellulose
- fasting before application: 15 - 20 h
- observation period: 14 days
- max. volume applied: 10 mL/kg bw.
- range of concentration: 2.0-0.5 % (g/v)

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 50 - < 200 mg/kg bw
Mortality:
Mortality in male rats after 1 hour/1 day/2 days/7 days/14 days:
- 200 mg/kg bw dose level: 5/5/5/5/5
- 50 mg/kg bw dose level: 0/0/0/0/0


Mortality in female rats after 1 hour/1 day/2 days/7 days/14 days:
- 200 mg/kg bw dose level: 5/5/5/5/5
- 50 mg/kg bw dose level: 1/1/1/1/1
Clinical signs:
- 200 mg/kg bw dose level: dyspnoe, apathy, abnormal position, staggering, atonia, no reflex on pain, convulsion, exsiccosis, poor general state.
- 50 mg/kg bw dose level: dyspnoe, apathy, abnormal position, staggering, tremor, convulsion, exsiccosis, poor general state.
Body weight:
Mean body weight [g] in male rats before application/2-4 days after appl./7 days after appl./13 days after appl.
- 464 mg/kg bw dose level:----
- 316 mg/kg bw dose level: 24/27.6/30.6/34.2


Mean body weight [g] in female rats before application/2-4 days after appl./7 days after appl./13 days after appl.
- 464 mg/kg bw dose level:----
- 316 mg/kg bw dose level: 24/25/27.5/28.3

Any other information on results incl. tables

Groups of 5 male and 5 female NMRI mice were given a single intraperitoneal 
injection of the test substance at dose levels of 50 and 200 mg/kg bw. For 
application, the test substance was suspended in a 0.5% aqueous preparation of 
carboxymethylcellulose; the suspensions were injected at 10 ml/kg bw. After 
dosing, the animals were observed for 14 days. One low dose female and all high 
dose animals died within one hour after dosing. Signs of toxicity were observed 
at both dose levels and included dyspnea, apathy, anomal position, staggering, 
atony, loss of pain reflex, narcotic-like state, tremor, spastic gait, rolling 
fitm, tonic convulsion, clonic convulsion, exsiccosis and poor general state. 
Body weight gains of the survivors were within the expected range. Post-mortem
 examination of the decedents and survivors revealed no pathological findings

Applicant's summary and conclusion