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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Similar to guideline study (OECD 402). Non-GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Principles of method if other than guideline:
BASF Test
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dimethoxyphenylacetonitrile
EC Number:
202-225-1
EC Name:
3,4-dimethoxyphenylacetonitrile
Cas Number:
93-17-4
Molecular formula:
C10H11NO2
IUPAC Name:
3,4-dimethoxyphenylacetonitrile
Test material form:
solid
Details on test material:
Veratrylcyanid, PSN 78/834; no data on purity given in the report.

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
- Source: Hagemann
- diet: Herilan MRH
- mean weights at application time: 203 g (females) and 258 g (males).

Administration / exposure

Type of coverage:
not specified
Vehicle:
water
Details on dermal exposure:
- way of application: dermal
- area: 42 cm2 on the back
- form of application: suspension
- formulation in: 50% aqua dest.
- observation period: 14 days
Duration of exposure:
24 h
Doses:
1,000 and 2,000 mg/kg bw.
No. of animals per sex per dose:
3
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: not described in the protocol
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Mortality:
no mortality observed.
Clinical signs:
Irregular respiration, apathy, staggering, spasms. Slight reversible erythema 24 h after the application.
Body weight:
Mean body weights: 258 g (males) and 203 g (females).
Gross pathology:
No abnormalities observed in the organs of any sacrificed animal.

Any other information on results incl. tables

LD50 > 2000 mg/kg bw.

A 50% aqueous formulation of the test substance was applied. After dosing, the rats were observed for 14 days. Clinical signs of toxicity included irregular respiration, apathy, staggering and spastic gait. Local (skin) effects were limited to slight primary skin irritation that was observed after 24 hours. No pathological changes were noted.

Applicant's summary and conclusion

Conclusions:
Based on the results of this study, the test substance veratrylcyanid does not need to be classified according to Regulation (EC) No. 1272/2008.