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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dimethoxyphenylacetonitrile
EC Number:
202-225-1
EC Name:
3,4-dimethoxyphenylacetonitrile
Cas Number:
93-17-4
Molecular formula:
C10H11NO2
IUPAC Name:
3,4-dimethoxyphenylacetonitrile
Details on test material:
- Name of test material (as cited in study report): 3,4-dimethoxyphenylacetonitrile (Veratrylcyanid krist. SF9)
- Lot/batch No.: 011924AX10
- Analytical purity: 98.6 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (125.3 mg/l) was prepared by stirring the test substance in deionized water for about 20 h at approximately 20 ± 2°C . The test concentrations were prepared by diluting the stock solution.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): Collection of algal cultures in Göttingen

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 ± 2 °C
pH:
8.0 - 9.4
Nominal and measured concentrations:
Nominal test substance concentrations:
1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/l

Analytically determined test concentrations:
1.28 mg/l (nominal 1.56 mg/l),
6.74 mg/l (nominal 6.25 mg/l),
25.8 mg/l (nominal 25 mg/l),
105.7 mg/l (nominal 100 mg/l)
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml)
- Initial cells density: 10000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test medium was prepared according EEC Directive 92/69/EEC, Annex V, Part C .3 .


OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light, type universal white (OSRAM L 25), about 60 - 120 µE/(m²s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber (Neubauer improved)
- Chlorophyll measurement: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm)

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
41.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

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