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EC number: 215-350-1 | CAS number: 1323-03-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
The category of alkyl lactates mainly contain data on propanoic acid, 2-hydroxy, C12-C16 (even and odd numbered) alkyl esters.
2. SOURCE AND TARGET CHEMICALS
Both the target and the analogues are produced by esterification of alcohols and lactate.
3. ANALOGUE APPROACH JUSTIFICATION
Since there is a significant relation between the analogues measured and the target substance, which only differs in chain length of the alcohol, read-across is here justified.
See section 13 for RA-Justification_Assessment report - Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Test Item Name Lactic Acid Dodecyl Ester
IUPAC Name Dodecyl lactate
CAS Number 6283-92-7
Batch/Lot Number 20150911001
Analysed Purity
(Genearted at JRF) 75.08%
Manufactured by Finetech Industries Limited
Supplied to JRF by Finetech Industry Limited, U.K.
Date of Expiry April 12, 2017
Date of Re-analysis April 12, 2017
Appearance Light yellow liquid - Analytical monitoring:
- yes
- Details on sampling:
- 50 mL of test samples from each replicates were drawn and mixed together for each group at 0 and 24 h at each renewal. For the concentration analysis of main stock solution, 100 mL of test sample from stock A1 was drawn at day 0 and day 1. The samples were divided into two equal portions. One portion (100 mL) was sent for test concentration analysis and the second portion (100 mL) was stored at -20 ± 5 ºC temperature till the study completion.
- Vehicle:
- no
- Details on test solutions:
- Preparation of Test concentrations:
On day 0, quantity of 200 mg lactic acid dodecyl ester were weighed in two replicate individually, and made up to 2000 with reconstituted water in two different glass bottle (stock A1 and A2, Test vessel of A1 and A2 were rinsed with test solution of 100 mg lactic acid dodecyl ester/L) to achieve the final concentration of 100% WAF prepared at 100 mg lactic acid dodecyl ester/L and kept for continuous stirring using magnetic stirrer for approximate 24 h at room temperature. After stirring, test solutions were kept for re-equilibrium for approximate 24 h. After phase separation, test solutions were collected from lower portion by “L” shaped glass tube without disturbing the phase. Volume of 1000 mL solution was collected from stock A1.
To achieve the final concentrations of 0.13, 0.28, 0.62, 1.36 and 3.0% WAF prepared at 100 mg lactic acid dodecyl ester/L,volume of 1.3, 2.8, 6.2, 13.6 and 30 mL from stock A1 were made upto 1000 mL with test media, respectively. Four replicates were used for each test concentration with 120.5 mL of volume in each test vessel. Prior to adding the volume of test solution used for exposure to the test vessels, test vessels were pre-conditioned with respective test concentrations to saturate the surface of the respective vessel to prevent loss of test concentration due to absorption at the walls at the test vessels. Same procedure was followed on day 1 for preparation of test concentration. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISMS:
- Master culture: procured from the Department of Zoology, University of Pune, India and regularly subcultured at the Ecotoxicology Laboratory of Jai Research Foundation.
- Sensitivity validtion: validation study with potassium dichromate as a positive control.
- Feeding: unicellular algae Pseudokirchneriella subcapitata during culturing and 48 h prior to experimentation.
- Age: The experiment was initiated with neonatal daphnids (less than 24 h old).
CULTURE CONDITIONS:
- mean temperature: 20.6 ± 0.1 °C,
- mean dissolved oxygen level: 8.97 ± 0.06 mg/L
- lIGHT/DARK CYCLUS: 16 h light and 8 h dark photoperiod.
- pH: 7.66 ± 0.06
- total hardness: 202.8 mg/L as CaCO3.
- Light Intensity: 1310 Lux. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 187.2 mg/L as CaCO3
- Test temperature:
- 20.7 - 21.5 °C
- pH:
- 7.50 - 7.79
- Dissolved oxygen:
- 8.47 - 8.81 mg/L
- Nominal and measured concentrations:
- WAF prepared at 100 mg/L (%) : 0.13 0.28 0.62 1.36 3 100
Concentration measured in fresh solutions at t=0h (mg/L) 0.0164 0.0414 0.0695 0.1342 0.3526 15.2
Concentration measured in fresh solutions at t=24H (mg/L) 0.0159 0.0436 0.0591 0.1442 0.3086 9.9
Average initial concentrations measured at t= 0h and 24h (mg/L) 0.01615 0.0425 0.0643 0.1392 0.3306
Time Weighed Average measured for the 48-h period (mg/L) 0.0151 0.0387 0.0551 0.0954 0.1895 - Details on test conditions:
- - Observations: All test daphnids were observed for immobility and abnormal behavior or appearance, if any, at 0, 24 and 48 h of exposure. Mobility of the daphnids was assessed by gently swirling the test container for 15 seconds and observing their swimming behaviour. Those daphnids unable to swim during the agitation of the test container and organisms remaining settled on the water surface or settled at the bottom of the test vessel were recorded as immobile.
- Test conditions: Temperature, dissolved oxygen and pH of the test media were measured using HQ40d (multimeter). Total hardness of the test media was measured using titrimetric method (IS 3025, 1983) prior to treatment.
- Photoperiod: 16 h light and 8 h darkness cycle.
- Other: The whole experiment was conducted in a specially designed low temperature water bath preset to a water temperature of 20.0 °C. Uniform temperature in the water chamber of the water bath was obtained by maintaining a slow flow of water by a motor. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95%-confidence interval: 0.085 - 0.12
- Details on results:
- The EC50 Value corresponded with 11.5% of a WAF prepared at 100 mg/L, which means that the EC50 was below the solubility limit of Ceraphyl 41.
- Results with reference substance (positive control):
- The 48h-EC50 of potassium dichromate was 0.67 mg/L with a 95%-confidence interval of 0.42 - 1.07 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions of this study, the EC50 of the test substance for Daphnia magna was below its solubility limit of approximately 6 mg/L, i.e. 48h-EC50 is 0.1 mg/L.
Reference
Lactic acid dodecyl ester concentration based on the analytical results:
Concentration:%WAF prepared at 100 mg Lactic Acid Dodecyl Ester/L |
Measured Concentration ofLactic Acid Dodecyl Esterat 0 day 0 h (mg/L) |
Measured Concentration ofLactic Acid Dodecyl Esterat 0 day 24 h (mg/L) |
Average Exposure Concentration ofLactic Acid Dodecyl Esterduring 24 h period (0 day) (mg/L) |
0.13 |
0.0164 |
0.0146 |
0.0155 |
0.28 |
0.0414 |
0.0362 |
0.0387 |
0.62 |
0.0695 |
0.0515 |
0.0598 |
1.36 |
0.1342 |
0.0654 |
0.0937 |
3.000 |
0.3526 |
0.0704 |
0.1576 |
Concentration:%WAF prepared at 100 mg Lactic Acid Dodecyl Ester/L |
Measured Concentration ofLactic Acid Dodecyl Esterat 1 day 0 h (mg/L) |
Measured Concentration ofLactic Acid Dodecyl Esterat 1 day 24 h (mg/L) |
Average Exposure Concentration ofLactic Acid Dodecyl Esterduring 24 h period (1 day) (mg/L) |
Concentration: Geometric mean measured concentration of lactic acid dodecyl ester at 48 h[[√(C0f x C24s)+ √(C24f x C48s)]/2 ] (mg/L) |
0.13 |
0.0159 |
0.0138 |
0.0148 |
0.0151 |
0.28 |
0.0436 |
0.0344 |
0.0387 |
0.0387 |
0.62 |
0.0591 |
0.0430 |
0.0504 |
0.0551 |
1.36 |
0.1442 |
0.0653 |
0.0970 |
0.0954 |
3.00 |
0.3086 |
0.1589 |
0.2214 |
0.1895 |
Immobility recorded in the 48 -h test with Daphnia magna exposed to different concentrations of lactic acid dodecyl ester :
Group |
Concentration: %WAF prepared at 100 mg Lactic Acid Dodecyl Ester/L |
Concentration: Geometric mean measured concentration of lactic acid dodecyl ester(mg/L) |
N° of Replicates |
N° of Daphnids/ group |
Immobility N° and % at |
||||
0 h |
24 h |
% |
48 h |
% |
|||||
G1 |
0.0 (Control) |
- |
4 |
20 |
0 |
0 |
0 |
0 |
0 |
G2 |
0.13 |
0.0151 |
4 |
20 |
0 |
0 |
0 |
0 |
0 |
G3 |
0.28 |
0.0387 |
4 |
20 |
0 |
0 |
0 |
0 |
0 |
G4 |
0.62 |
0.0551 |
4 |
20 |
0 |
0 |
0 |
2 |
10 |
G5 |
1.36 |
0.0954 |
4 |
20 |
0 |
1 |
5 |
7 |
35 |
G6 |
3.0 |
0.1895 |
4 |
20 |
0 |
3 |
15 |
19 |
95 |
Description of key information
Value based on a GLP study with the analogue dodecyl lactate supported by EC50 values estimated by trend analysis or ECOSAR v. 1.1 (QSAR).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.36 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 0.05 mg/L
Additional information
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