Sodium [4-[[dihydroxy[(2-hydroxy-3,5-dinitrophenyl)azo]phenyl]azo]benzenesulphonato(3-)]cuprate(1-)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Nov 10, 2016 - Jan 11, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Version / remarks:

OECD 209:2010

Deviations:

no

GLP compliance:

yes

Test organisms (species):

activated sludge

Details on inoculum:

- Source of inoculum/activated sludge: Dihua sewage treatment plant, Taiwan.

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 ± 2 °C

pH:

6.91-7.65

Nominal and measured concentrations:

3.91, 15.63, 62.50, 250.00 and 1000.00 mg/L

Details on test conditions:

- Aeration: The test mixture was incubated under conditions of forced aeration at 0.5-1.0 L/min with air removed dust and oil.
- No. of vessels per concentration (replicates): five
- No. of vessels per reference and blank control (replicates): five
- Sludge concentration: 1.50 g/L
- Nitrification inhibitor used : N-allylthiourea

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Table 1. Oxygen consumption rates of test group for the definitive test

Group (mg/L)	Oxygen consumption rate (mg/Lh)	Average	Coefficient of variation (%)
Blank control group	63.00	61.80	10.34
	62.52
	71.40
	57.24
	54.84
Nitrification control group	42.84	─	─
Test group
3.91	63.96	57.17	10.86
	53.28
	50.52
	63.60
	54.48
15.63	60.84	59.16	12.09
	53.52
	53.28
	57.48
	70.68
62.50	45.96	51.24	9.52
	57.24
	48.60
	55.56
	48.84
250.00	47.64	47.78	11.66
	52.68
	48.12
	51.84
	38.64
1,000.00	33.96	36.98	9.09
	40.56
	34.32
	40.68
	35.40

Table 2. Oxygen consumption rates ofreference controlgroups for the definitive test

Group (mg/L)	Oxygen consumption rate (mg/Lh)	Average	Coefficient of variation (%)
Blank control group	48.72	40.92	11.63
	36.36
	41.76
	38.88
	38.88
Nitrification control group	34.44	─	─
Reference control group
0.39	40.44	34.78	13.98
	28.68
	33.24
	39.00
	32.52
1.56	36.36	30.79	37.75
	18.60
	37.80
	18.12
	43.08
6.25	25.56	26.47	22.19
	36.00
	24.24
	26.52
	20.04
25.00	9.60	9.17	12.37
	8.52
	9.12
	10.80
	7.80
100.00	8.28	8.02	42.83
	9.36
	12.48
	6.84
	3.12

Table 3. Inhibition rates of total oxygen uptake and EC₅₀ for the definitive test

Group (mg/L)	Average inhibition rate of total oxygen uptake (%)	EC50 (mg/L)
Test group
3.91	7.50	>1000.00
15.63	4.27
62.50	17.09
250.00	22.68
1000.00	40.16
Reference control group
0.39	15.01	8.06
1.56	24.75
6.25	35.31
25.00	77.60
100.00	80.41

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 209 test method, the EC50 of Everlan SL65 for oxygen consumption rate of sludge was greater than 1000 mg/L.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100109001EN which is based on the SOP for the OECD 209 (SOPE-508) and OECD 209 (OECD, 2010). The results of this OECD 209 test for Everlan SL65 show that all validity criteria were met. The EC50 of Everlan SL65 for oxygen consumption rate of sludge was greater than 1000 mg/L.

 

Description of key information

The EC₅₀ of Everlan SL65 for oxygen consumption rate of sludge was greater than 1000 mg/L (OECD TG 209).

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

For blank control groups at the end of the test, the coefficient of variation of oxygen consumption rate were less than 30% and the oxygen consumption rate greater than 20.00 mg/h of per one gram of sludge, respectively. The EC50 of reference control group (3,5-Dichlorophenol) was lied in the range between 2.00 and 25.00 mg/L. All test validity criteria were met. The EC50 of Everlan SL65 for oxygen consumption rate of sludge was greater than 1000 mg/L.