Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 1995 - September 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: ASTM E 729-780. Standard practice for conducting toxicity tests with fishes, macroinvertebrates, and amphibians
Version / remarks:
1980
Qualifier:
according to guideline
Guideline:
other: OECD 202
Version / remarks:
1981
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material: Everlan Brown EFR
Analytical monitoring:
yes
Test organisms (species):
Cyprinus carpio
Details on test organisms:
- Common name: Common carp
- Source: Wu Sha Tow Fresh Water Fish Culture Demonstration Center, R.O.C.
- Age at study initiation: 60-90 days old
- Length at study initiation: 2-4 cm
- Acclimation period: at least seven days
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
pH:
8.4-8.5
Dissolved oxygen:
73-81%
Details on test conditions:
- Test vessel: Glass tank, 23 cm (id) × 30 cm (h)
- Test volume: 10 L
- Aeration: Gently
- Renewal rate of test solution: 24 hours
- Photoperiod: 12-hours light
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
5.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

Table 1. Water quality measurements during the acute toxicity test of Everlan Brown EFR to common carp (Cyprinus carpio)

Nominal concentration

(mg/l)

Exposure Time (hr)

3

24

48

72

96

pH

10

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.0

8.5 ± 0.0

8.4 ± 0.0

6.0

8.4 ± 0.0

8.5 ± 0.0

8.4 ± 0.0

8.5 ± 0.0

8.5 ± 0.0

3.6

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.0

8.5 ± 0.0

2.2

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.1

8.5 ± 0.0

1.3

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.0

8.5 ± 0.0

8.4 ± 0.0

Control

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.0

8.4 ± 0.1

8.5 ± 0.0

Dissolved oxygen, % saturation (mg/l)

10

76 (6.3 ± 0.0)

79 (6.5 ± 0.1)

73 (6.0 ± 0.2)

75 (6.2 ± 0.0)

81 (6.7 ± 0.0)

6.0

75 (6.2 ± 0.1)

76 (6.3 ± 0.2)

75 (6.2 ± 0.1)

77 (6.4 ± 0.2)

79 (6.5 ± 0.1)

3.6

75 (6.2 ± 0.0)

77 (6.4 ± 0.1)

75 (6.2 ± 0.1)

76 (6.3 ± 0.0)

77 (6.4 ± 0.2)

2.2

73 (6.0 ± 0.1)

77 (6.4 ± 0.2)

76 (6.3 ± 0.2)

77 (6.4 ± 0.1)

76 (6.3 ± 0.4)

1.3

75 (6.2 ± 0.2)

77 (6.4 ± 0.2)

77 (6.4 ± 0.1)

77 (6.4 ± 0.0)

77 (6.4 ± 0.2)

Control

76 (6.3 ± 0.0)

75 (6.2 ± 0.1)

76 (6.3 ± 0.0)

79 (6.5 ± 0.2)

80 (6.6 ± 0.2)

mg/l calcium as CaCO3 (electrode potential, mV)

10

100 (16 ± 0)

109 (17 ± 0)

109 (17 ± 0)

109 (17 ± 0)

109 (17 ± 0)

6.0

100 (16 ± 0)

109 (17 ± 0)

109 (17 ± 0)

119 (18 ± 0)

109 (17 ± 0)

3.6

109 (17 ± 0)

109 (17 ± 0)

109 (17 ± 0)

119 (18 ± 0)

109 (17 ± 0)

2.2

109 (17 ± 0)

119 (18 ± 0)

109 (17 ± 0)

109 (17 ± 0)

109 (17 ± 0)

1.3

100 (16 ± 0)

119 (18 ± 0)

109 (17 ± 0)

109 (17 ± 0)

109 (17 ± 0)

Control

109 (17 ± 0)

129 (19 ± 0)

119 (18 ± 0)

109 (17 ± 0)

119 (18 ± 0)

Table 2. Survival of common carp (Cyprinus carpio) exposed to Everlan Brown EFR for 96 hours

Nominal Concentration

(mg/L)

No. of Survival Fish

0 hr

3 hr

24 hr

48 hr

72 hr

96 hr

10

30

30

3

2

0

0

6.0

30

30

21

19

18

16

3.6

30

30

27

22

0

0

2.2

30

30

30

30

30

30

1.3

30

29

29

29

29

29

Control

30

30

30

30

30

30

Table 3. The acute toxicity of Everlan Brown EFR to common carp (Cyprinus carpio)

Exposure Time

(hr)

LC50

(mg/l)

95% Confidence (mg/l)

Lower

Upper

3

>100

 

 

24

7.0

5.9

8.3

48

5.9

5.1

7.0

72

5.8

5.0

6.8

96

5.6

4.8

6.6

Validity criteria fulfilled:
yes
Conclusions:
According to ASTM E 729-780 and OECD 202 test method, the 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L.
Executive summary:

This test using the procedures outlined in the TACTRI Study Plan for EF3G0083.5 (95AQC212) which is based on the ASTM E 729-780 (ASTM, 1980) and OECD 202 (OECD, 1981). The values of water quality measurements were considered adequate for testing. There was no significant symptom of toxicity noted in this study. Under the conditions of this test, the 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L.

Description of key information

The 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L for common carp (ASTM E 729-780 and OECD TG202).

 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
5.6 mg/L

Additional information

The values of water quality measurements contain pH (8.4-8.5), dissolved oxygen concentrations (73%-81% of air saturated value), and total hardness (110-1299 mg/L as CaCO3) were considered adequate for testing. There was no significant symptom of toxicity noted in this study. Under the conditions of this test, the 96-hr LC50 of Everlan Brown EFR was 5.6 mg/L.