Sodium [4-[[dihydroxy[(2-hydroxy-3,5-dinitrophenyl)azo]phenyl]azo]benzenesulphonato(3-)]cuprate(1-)

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 15, 2016 - Dec 06, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

OECD 121:2001

Deviations:

no

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Details on study design: HPLC method:

- Apparatus: Agilent-1200 Series High Performance Liquid Chromatography
- Type, material and dimension of analytical (guard) column: BDS HYPERSIL Cyano Column, 5μm, 150 × 4.6 mm (Thermo)
- Detection system: Agilent-1260 Infinity Photodiode Array Detector

Computational methods:

Aglient ChemStation Rev. B.0401 electronic data software was used for HPLC/PDA control sysytem, data collection and data processing.

Key result

Type:

log Koc

Remarks:

on sewage suldge

Value:

1.97

Key result

Type:

log Koc

Remarks:

on soil

Value:

2.37

Table 1: The logarithm of adsorption coefficient (log Koc) on sewage sludge and on soil and the logarithm of capacity factor (log K’) of control article and reference article.

Article Name	Log Koc		Retention time (min)		Average retention time (min)	Capacity factor (K’)	Log K’
	Sewage sludge	Soil	t1	t2
Control article
Formamide	-	-	3.021	3.028	3.025	NA	NA
Reference article
Acetanilide	1.52	1.26	4.924	4.931	4.928	0.629	-0.201
Atrazine	1.66	1.81	8.005	8.005	8.005	1.647	0.217
Monuron	1.46	1.99	7.660	7.664	7.662	1.533	0.186
Phenanthrene	4.35	4.09	13.525	13.519	13.522	3.471	0.540
Benzonic acid phenylester	3.26	2.87	11.484	11.473	11.479	2.795	0.446
Fenthion	3.75	3.31	13.957	13.952	13.955	3.614	0.558
2,5-dichloroaniline	2.45	2.55	8.580	8.579	8.580	1.837	0.264

NA: Not application

Table 2: Retention time and capacity factor (K’) of test article Everlan SL65.

Peak	Retention time (min)		Average retention time	Average capacity factor (K’)	Log average capacity factor (log K’)
	t1	t2
1	8.411	8.394	8.403	1.778	0.250

Table 3: The adsorption coefficient (Koc) on sewage sludge and on soil of test article Everlan SL65.

Peak	The logarithm of adsorption coefficient (log KOC)		The adsorption coefficient (KOC)		Area of peak (%)
	Sewage sludge^a	Soil^b	Sewage sludge	Soil
1	1.97	2.31	93.54	205.13	100.00

a: Calibration curve of reference article on sewage sludge was y=0.139x – 0.024, R²= 0.939.

b: Calibration curve of reference article on soil was y=0.330x – 0.513, R²= 0.898

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 121 test method, the adsorption coefficients (KOC) of Everlan SL65 on sewage sludge and on soil were 93.54 and 205.13, respectively.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100110001EN which is based on the SOP for the OECD 121 (SOPE-507) and OECD 121 (OECD, 2001). The results of this OECD 121 test for Everlan SL65show that all validity criteria were met. The adsorption coefficients (K_OC) of Everlan SL65 on sewage sludge and on soil were 93.54 and 205.13, respectively.

Description of key information

Respectively, the Log adsorption coefficients (Log K_OC) of Everlan SL65 on sewage sludge and on soil were 1.97 and 2.31 (OECD TG 121). The test is not done in the water. The estimated Log Koc water is 2.0.

Key value for chemical safety assessment

Koc at 20 °C:

100

Other adsorption coefficients

Type:

log Kp (solids-water in activated sewage sludge)

Value in L/kg:

1.97

at the temperature of:

20 °C

Other adsorption coefficients

Type:

log Kp (solids-water in soil)

Value in L/kg:

2.31

at the temperature of:

20 °C

Additional information

All of the articles were ran in duplicate and the difference of log K_OC of test article between duplicate were < 0.25. In order to confirm retention times of test article had not drifted on HPLC analysis, formamide as the control article was analyze before and after the test artic. All test validity criteria were met. The adsorption coefficients (K_OC) of Everlan SL65 on sewage sludge and on soil were 93.54 and 205.13, respectively.

[LogKoc: 2.0]