Registration Dossier
Registration Dossier
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EC number: 219-546-8 | CAS number: 2459-09-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Inverse correlation between combined mutagenicity in Salmonella typhimurium and strength of coordinate bond in mixtures of cobalt (II) chloride and 4-substituted pyridines
- Author:
- H. Iyehara Ogawa , Song-Yuan Liu , Kazunori Sakata , Yoshiyuki Niyitani ,Satoshi Tsuruta 2 and Yasuhiko Kato
- Year:
- 1 988
- Bibliographic source:
- Mutation Research, 204 (1988) 117-121
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of Methyl Isonicotinate in Salmonella typhimurium LT2 strains TA1537, TA2637, TA97a, TA98 and TA100 by Bacterial reverse mutation assay.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl isonicotinate
- EC Number:
- 219-546-8
- EC Name:
- Methyl isonicotinate
- Cas Number:
- 2459-09-8
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- methyl pyridine-4-carboxylate
- Details on test material:
- - Name of test material (as cited in study report): Methyl isonicotinate
- Molecular formula (if other than submission substance): C7H7NO2
- Molecular weight (if other than submission substance): 137.1373 g/mole
- Substance type: Organic
- SMILES:COC(=O)c1ccncc1
- InChI: 1S/C7H7NO2/c1-10-7(9)6-2-4-8-5-3-6/h2-5H,1H3
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Methyl isonicotinate
- Molecular formula (if other than submission substance): C7H7NO2
- Molecular weight (if other than submission substance): 137.1373 g/mole
- Substance type: Organic
- SMILES:COC(=O)c1ccncc1
- InChI: 1S/C7H7NO2/c1-10-7(9)6-2-4-8-5-3-6/h2-5H,1H3
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA1537, TA2637, TA97a, TA98 and TA100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- not specified
- Vehicle / solvent:
- not specified
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation) - Rationale for test conditions:
- not specified
- Evaluation criteria:
- Number of Histidine revertants per plate was observed.
- Statistics:
- not specified
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA1537, TA2637, TA98 and TA100
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: No mutagenic effect were observed
Applicant's summary and conclusion
- Conclusions:
- Methyl Isonicotinate (2459-09-8) was evaluated for its mutagenic potential in Salmonella typhimurium strains TA1537, TA2637, TA98 and TA100. The test result was considered to be negative for Bacterial reverse mutation assay.
- Executive summary:
In Vitro genetic toxicity study for Methyl Isonicotinate was assessed for its possible mutagenic potential. For this purpose Bacterial reverse mutation assay was performed in Salmonella typhimurium strains TA1537, TA2637, TA98, TA97a and TA100. Plate incorporation method was used. The examination with TA97a was unsuccessful because bacteria did not grow on Tris minimal agar.No mutagenic effects were observed in all other strain.. Therefore Methyl Isonicotinate was considered to non mutagenic in Salmonella typhimurium strains TA1537, TA2637, TA98 and TA100. Hence the substance cannot be classified as gene mutant in vitro.
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