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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Description of key information

Short-term toxicity

In a 14-day acute toxicity study (IBACON, 394408, 2011) with Eisenia fetida according to OECD 207 (1984), the LC50 of the test substance with Eisenia fetida was > 1000 mg/kg dry soil.

 

Long-term-toxicity

In a 56 day chronic test (BASF SE, 394409, 2012) with Eisenia fetida according to OECD 222 (2004) the test substance caused no adverse effects on survival, biomass development and reproduction of parent earthworms up to and including the highest concentration of 473 mg/kg dry soil. The overall NOEC for weight change, survival and reproduction was equivalent to 473 mg/kg soil dry weight. The EC50 for reproduction is 804.39 mg/kg soil dry weight (> highest concentration tested).

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
473 mg/kg soil dw

Additional information

Short-term toxicty

In a 14-day acute toxicity study (IBACON, 394408, 2011) with Eisenia fetida, earthworms were exposed to the test substance according to OECD 207 (1984) for 14 days in artificial soil (OECD 207 substrate with 5% organic matter). The test was conducted in 1 L glass bottle jars covered with a glass lid, containing 500 g artificial dry soil. The untreated control and nominal test item concentrations of 198, 296, 444 667 and 1000 mg/kg dry soil were tested with 4 replicates containing 10 worms each. The vessels were maintained at 18.0 - 22.0 °C under continuous light.  After 14 days of exposure, no mortality was observed in the control or any test item concentration. The body weight changes were not statistically significantly different compared to the control up to and including the highest concentration of 1000 mg/kg dry soil. No behavioral effects were observed. The LC50 of the test substance with Eisenia fetida was > 1000 mg/kg dry soil.

 

In addition in a 14 -d toxicity study (BioChem agrar, 394877, 2014) to earthworms (Eisenia fetida) according to OECD 207 (1984) with the metabolite (M440I002) of the registered test substance, the LC50 was estimated to be greater than 1000 mg/kg soil dry weight.

 

Moreover in a 14-day acute toxicity study (IBACON, 394871, 2014) according to OECD 207 (1984) with the metabolite (M440I003) of the registered test substance to earthworms (Eisenia fetida) the 14-day LC50 was determined to be greater than 1000 mg test item/kg dry soil.

 

Additionally in a 14-d toxicity study (Labor für biologische und chemische Analytik GmbH, 394883, 2014) to earthworms (Eisenia fetida) with the metabolite (M440I005) of the registered test substance the LC50 was estimated to be greater than 1000 mg/kg soil dry weight.

 

Additionally in a 14-d toxicity study (IBACON, 735863, 2015) to earthworms (Eisenia fetida) with the metabolite (M440I024) of the registered test substance the LC50 was estimated to be greater than 1000 mg/kg soil dry weight. The NOEC was determined to be ≥ 1000 mg /kg soil dry weight.

 

Long-term-toxicity

In a 56 day chronic test (BASF SE, 394409, 2012), earthworms (Eisenia fetida) were exposed to the test substance according to OECD 222 (2004) for 28 days in artificial soil (OECD 222 substrate with 5% organic matter). After this period, the adults were removed and cocoons remained in the test vessels for an additional 28 days. The test was conducted in1 L plastic dishes covered with transparent and punctured lids, containing 600 g artificial dry soil. Soil concentrations of29.54, 59.1, 118, 236 and 473 mg/kg dry soil (corresponding to nominal concentrations of 31.25, 62.5, 125, 250 and 500 mg/kg dry soil) were tested in 4 replicates of 10 worms each. Additionally, an untreated control with 8 replicates each containing 10 worms was included. The vessels were maintained at 19 – 21 °C under a 16 hours light (400-800 lux) and 8 hours dark photoperiod. Soil moisture was maintained at 60% of the WHC. No mortality of parent earthworms was observed in the control and any of the treatment groups up to and including the highest concentration of 473 mg/kg soil dry weight. Body weight change of the earthworms exposed to the test substance was not statistically significantly different compared to the control up to and including the highest test item concentration of 473 mg/kg soil dry weight. The feeding activity of the earthworms was not reduced up to and including the highest concentration of 473 mg per kg soil dry weight. No other behavioral abnormalities were observed in any of the treatment groups. Reproduction was not statistically different compared to those in the control up to and including the highest test concentration of 473 mg/kg soil dry weight. The overall NOEC for weight change, survival and reproduction was equivalent to 473 mg/kg soil dry weight. The EC50 for reproduction is 804.39 mg/kg soil dry weight (> highest concentration tested).