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Short-term toxicity to aquatic invertebrates

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Description of key information

Freshwater

 in a 48-h static supporting acute toxicity study (Eco-Science Corporation, SG-ES2011-B05D, 2011) with Daphnia magna the EC50 of the test substance was determined to be 8.0 mg/L (mean measured).

Marine water:

In a 96-hour acute toxicity laboratory key study (Wildlife International, 394392, 2012) according to EPA OPPTS 850.1025 (Bivalve Acute Toxicity (shell deposition test), 1996) the effect of the test substance on shell deposition of eastern oysters (Crassostrea virginica) was investigated. The 96-hour EC50 based on comparison to the negative control was calculated to be 2.17 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
8 mg/L

Marine water invertebrates

Marine water invertebrates
Effect concentration:
2.17 mg/L

Additional information

Freshwater

In a 48-h static acute toxicity laboratory study (Eco-Science Corporation, SG-ES2011-B05D, 2011), neonates (< 24 h old) of Daphnia magna were exposed to nominal concentrations of 0.22, 0.49, 1.1, 2.3, 5.2, 11 and 25 mg/L, corresponding to mean measured concentrations of 0.23, 0.53, 1.3, 2.5, 5.8, 11 and 28 mg/L, respectively. The study included also a negative control and a solvent control group. The daphnids were tested in groups of 20 neonates per test concentration and control group, divided in groups of 5 in four 200-mL glass vessels, containing 100 mL test solution. Daphnids were observed for immobility and signs of toxicity at 24 hours and 48 hours after exposure. The EC50 of the test substance was determined to be 8.0 mg/L (mean measured).

Marine water:

In a 96-hour acute toxicity laboratory key study (Wildlife International, 394392, 2012) according to EPA OPPTS 850.1025 (Bivalve Acute Toxicity (shell deposition test), 1996) the effect of the test substance on shell deposition of eastern oysters (Crassostrea virginica) was investigated under flow-through conditions. The oysters were exposed to nominal concentrations of 0.63, 1.3, 2.5, 5.0 and 10 mg/L, corresponding to mean measured concentrations of 0.59, 1.2, 2.4, 4.5 and 7.3 mg/L, respectively. The study included also a negative and a solvent control. The animals were tested in groups of 20 oysters in one test chamber per treatment and control group containing filtered seawater. The oysters were observed for mortality and signs of toxicity after 1, 24, 48, 72 and 96 hours of exposure. Measurements of shell deposition for each oyster were made after 96 hours.

The biological results were based on mean measured concentrations. After 96 hours of exposure there were no mortalities among oysters in any treatment or control group. All oysters in the negative control, solvent control, 0.59, 1.2 and 2.4 mg/L treatment groups appeared normal throughout the 96-hour exposure period. Signs of toxicity were observed in the 4.5 and 7.3 mg/L treatment groups. Oysters in the 4.5 mg/L treatment group were observed as closed, and oysters in the 7.3 mg/L treatment group were observed as closed with a lack of fecal matter in the test chamber, which suggests abstinence from feeding. After 96 hours, the mean shell deposition in the negative and solvent control groups was 3.2 and 2.3 mm, respectively. There was statistically significant differences between shell deposition data for the negative control and the solvent control. Therefore, the treatment group data was compared to the both the solvent and negative control data. Mean shell deposition in the 0.59, 1.2, 2.4, 4.5 and 7.3 mg/L treatment groups was 3.0, 2.3, 1.4, 0.13 and 0.01 mm, respectively. Inhibition of shell growth in the 0.59, 1.2, 2.4, 4.5 and 7.3 mg/L treatment groups was -29, 1.1, 38.4, 94.5 and 99.8 %, respectively, when compared to the solvent control. When compared to the negative control, inhibition of shell growth in the 0.59, 1.2, 2.4, 4.5 and 7.3 mg/L treatment groups was 6.3, 28.2, 55.3, 96.0 and 99.8 %, respectively. There was a statistically significant decrease in shell deposition in the 4.5 and 7.3 mg/L treatment groups in comparison to the solvent control, and there was a statistically significant decrease in shell deposition in the 2.4, 4.5, and 7.3 mg/L treatment groups in comparison to the negative control.

The 96-hour EC50 based on comparison to the negative control group was calculated to be 2.17 mg/L.

In addition in a static supporting acute toxicity laboratory study (Wildlife International, 394390, 2015) with saltwater mysids (Americamysis bahia), the LC50 (96 h) for the test substance was determined to be 4.4 mg/L based on mean measured concentrations.