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EC number: 214-478-5 | CAS number: 1132-61-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 May - 18 June 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-morpholinopropanesulphonic acid
- EC Number:
- 214-478-5
- EC Name:
- 4-morpholinopropanesulphonic acid
- Cas Number:
- 1132-61-2
- Molecular formula:
- C7H15NO4S
- IUPAC Name:
- 3-morpholin-4-ylpropane-1-sulfonic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): 3-morpholin-4-ium-4-ylpropane-1-sulfonate
- Physical state : white powder
- Analytical purity : 99%
- Purity test date : not stated
- Lot/batch No. : 03100048/310
- Expiration date of the lot/batch : 2015-04-12
- Storage condition of test material : in a tightly closed vessel at room temperature (20 ±5 °C) under dry conditions in the dark
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: activated sludge, mostly domestic
- Details on inoculum:
- - Source of inoculum/activated sludge : The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf , Germany. Date of collection : 16 May 2014 , batch no: 20140516
- Pretreatment : The sludge was filtrated , washed with tap water twice , then washed with and re-suspended in test medium . It was then aerated for until use. The dry matter was determined as 4440 mg suspended solids/liter . - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 20 mg/L
- Based on:
- other: Carbon
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Preparations
The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its TOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined. The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2.
Experimental Parameters
Flask volume : 1500 mL
Apparatus blanks : 2, containing mineral medium only
Controls : 2, containing mineral medium and inoculum
Positive control flasks : 2, containing positive control, mineral medium and inoculum
Test flasks : 2, containing test item, mineral medium and inoculum
Abiotic control : 1, containing test item, mineral medium and HgCl2
Toxicity control : 1, containing test item, positive control, mineral medium and inoculum
Inoculum concentration : 24.9 mg/L
Temperature : 21.4 – 24.0 °C
Duration : 28 days
The test was performed with a nominal start concentration of 20 mg organic carbon/L.
The following amounts of test item and reference item were added to the flasks: see table 1 at "any other information on materials and methods incl. tables"
Apparatus
The test vessels were aerated with purified (by activated charcoal), CO2-scrubbed, moistened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5 m-NaOH. To control the absence of CO2, the air was then led through a flask containing a solution of Ba(OH)2 before reaching the test vessels. Magnetic stirrers were used to prevent deposition of inoculum. The emitted CO2 was trapped in 0.25-m-NaOH. Two scrubbers containing 100 ml each were connected in series to the test vessels. The initial IC value of the 0.25 m-NaOH was separately determined in each flask.
Sampling
From each front scrubber flask, ten samples were taken in order to determine the emitted CO2 (on days 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29). The sample volume was 1 mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO2. On day 28, 5 mL HCl 2-m were added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken.
CO2 determination
Analyses of the emitted CO2 were made by IC measurement using the carbon analyser TOC multi N/C 2100S, Analytik Jena. Each sample was measured at least in duplicate. The carbon analyser was calibrated with freshly prepared reference solutions containing potassium hydrogen phthalate (TC), sodium hydrogen carbonate and sodium carbonate (IC) once a week. After every start, quality control samples were measured.
Reference substance
- Reference substance:
- aniline
- Remarks:
- 2.1 g/l
Results and discussion
- Test performance:
- All validity criteria were met.
Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation was not observed. Both replicates of the test item showed very good correspondence.
As degradation in the toxicity flask was 35 % after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 51.7 mg/L”.
Ready degradability is defined in the guidelines as degradation surpassing 60% within 10 days after reaching a level of 10%. Therefore, the test item 3-morpholin-4-ium-4-ylpro-pane-1 -sulfonate cannot be considered as “readily biodegradable”. Degradation missed 60% within 28 days, too. Therefore, the test item is not considered as “biodegradable within 28 days”, either.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- The following data were determined for the test item 3-morpholin-4-ium-4-ylpropane-1 - sulfonate:
10-day-window : not detected
degradation at the end of 10-day-window : none
degradation at the end of the test : 1 %
pass level following guideline : 60% at the end of 10-day-window
BOD5 / COD results
- Results with reference substance:
- Degradation of the reference item (Aniline) was 65 % after nine days.
Any other information on results incl. tables
Table 1 : Emitted Carbon (in mg/L)
Day | Control 1 | Control 2 | Positive Control 1 | Positive Control 2 | Test 1 | Test 2 | Abiotic Control | Toxicity Control |
2 | 0,27 | 0,62 | 0,28 | 0,39 | 0,45 | 0,70 | -0,01 | 0,50 |
4 | 0,79 | 1,04 | 3,60 | 5,63 | 1,31 | 1,17 | 0,01 | 4,37 |
7 | 1,19 | 1,62 | 9,97 | 11,22 | 2,10 | 1,77 | 0,10 | 10,39 |
9 | 1,74 | 2,44 | 15,50 | 15,12 | 2,73 | 2,33 | 0,06 | 13,93 |
11 | 2,03 | 2,73 | 16,25 | 16,14 | 3,10 | 2,73 | 0,19 | 14,75 |
14 | 2,06 | 2,73 | 16,20 | 16,31 | 2,81 | 2,93 | 0,15 | 16,53 |
18 | 2,40 | 3,36 | 18,56 | 18,84 | 3,54 | 3,45 | 0,21 | 18,94 |
23 | 2,41 | 3,37 | 18,11 | 17,35 | 3,31 | 3,35 | 0,19 | 18,45 |
29 | 2,86 | 4,35 | 19,73 | 18,79 | 3,86 | 3,71 | 0,12 | 19,68 |
Table 2 : Degradation-Values in %
Day | Positive Control 1 | Positive Control 2 | Positive Control Mean | Test 1 | Test 2 | Test Mean | Abiotic Control | Toxicity Control |
2 | -0,8 | -0,2 | -0,5 | 0,1 | 1,3 | 0,7 | 0,0 | 0,1 |
4 | 13,2 | 23,2 | 18,2 | 2,0 | 1,3 | 1,6 | 0,1 | 8,6 |
7 | 42,1 | 48,2 | 45,1 | 3,4 | 1,8 | 2,6 | 0,5 | 22,2 |
9 | 65,9 | 64,0 | 65,0 | 3,2 | 1,2 | 2,2 | 0,3 | 29,3 |
11 | 68,1 | 67,6 | 67,9 | 3,6 | 1,7 | 2,7 | 1,0 | 30,6 |
14 | 67,9 | 68,4 | 68,1 | 2,1 | 2,7 | 2,4 | 0,7 | 35,0 |
18 | 77,1 | 78,5 | 77,8 | 3,3 | 2,9 | 3,1 | 1,0 | 39,8 |
23 | 74,8 | 71,0 | 72,9 | 2,1 | 2,3 | 2,2 | 0,9 | 38,5 |
29 | 79,2 | 74,6 | 76,9 | 1,3 | 0,5 | 0,9 | 0,6 | 39,8 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria were met
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The study was performed according to OECD 301B/EU C.4-C without deviations and therefore considered to be of the highest quality (reliability Klimisch 1).
The test item 3-morpholin-4-ium-4-ylpropane-1-sulfonate was tested using a concentration of nominally 20 mg organic carbon/L (corresponding to 51.5 mg 3-morpholin-4-ium-4- ylpropane-1-sulfonate/L) in test medium.
Aniline was chosen as positive control.
Activated sludge was used as inoculum (concentration in the test 24.9 mg dry matter/L). The test was left running for 28 days.
All validity criteria were met. Degradation of the positive control was 65 % after nine days.
The following data were determined for the test item 3-morpholin-4-ium-4-ylpropane-1 - sulfonate:
10-day-window : not detected
degradation at the end of 10-day-window : none
degradation at the end of the test : 1 %
pass level following guideline : 60% at the end of 10-day-window
Therefore, regardless of the 10-day-window, 3-morpholin-4-ium-4-ylpropane-1-sulfonate is not biodegradable following OECD 301 B/EU C.4-C. - Executive summary:
The biodegradability of the substance 3-morpholin-4-ium-4-ylpropane-1-sulfonate was investigated according to OECD guideline 301B and EU method C.4 –C. The study was conducted in compliance with the Principles of Good Laboratory Practice (GLP). Activated sludge collected from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, NW-Lachen-Speyerdorf, Germany, which treats predominantly domestic sewage was used as inoculum. The test item was tested using a concentration of 20 mg organic carbon/L (corresponding to 51.5 mg 3-morpholin-4-ium-4- ylpropane-1-sulfonate/L) in test medium following OECD 301B/EU-Guideline C.4-C. Aniline was chosen as positive control. The test was left running for 28 days. Analyses of the emitted CO2 were made by IC measurement using the carbon analyzer TOC multi N/C 2100S, Analytik Jena. All validity criteria were met. Degradation of the positive control was 65% after nine days.
The following data could be determined for the test item 3-morpholin-4-ium-4-ylpropane-1-sulfonate :
10-day-window : not detected
degradation at the end of 10-day-window : none
degradation at the end of the test : 1 %
pass level following guideline : 60% at the end of 10-day-window
Therefore, regardless of the 10-day-window, 3-morpholin-4-ium-4-ylpropane-1-sulfonate is not biodegradable following OECD 301 B/EU C.4-C.
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