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EC number: 429-600-4 | CAS number: 1026988-42-0
Standard Plate Test (SPT):
Tests without S-9 mix: TA1535, TA 100, TA1537, TA98, E. coli WP2 uvrA: No increase in the number of his+ or trp+ revertants.
Tests with S-9 mix: TA1535, TA 100, TA1537, TA98, E. coli WP2 uvrA: No increase in the number of his+ or trp+ revertants.
Preincubation Test (PIT):
Test with S-9 mix: TA 1535, TA98: No increase in the number of his+ revertants; TA 100: Slight increase in the number of his+ revertants over a dose range of about 500 µg - 1500 µg/plate (factor 1.3 - 2.3); TA 1537: increase in the number of his+ revertants from about 500 µg- 750 µg/plate (factor 2.4 - 3.0 onward with a maxium at 1000 µg- 1250 µg/plate (factor 3.6 -4.4); E.coli WP2 uvrA: Weakly positive reaction of about 500µg/plate (factor 2.0).
A bacteriotoxic effect (reduced his+ or trp+ background growth, decrease in the number of his+ or trp+ revertants, reduction in the titer) was observed depending on the strain and test conditions at doses from about 500 µg - 2500 µg/plate onward (SPT).
In the preincubation assay bacteriotoxicity was found without S-9 mix from about 100µg - 500µg/plate onward (all tester strains) or with S-9 mix depending on the strain at doses >= 1,000µg/plate (TA 1535, TA1537, TA 98, E. coli WP2 uvrA).
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