Pyridinium, 1,1'-[(6,13-dichloro-4,11-disulfo-3,10-triphenodioxazinediyl)bis[imino-2,1-ethanediylimino[6-[(2,5-disulfophenyl)amino]-1,3,5-triazine-4,2-diyl]]]bis[3-carboxy-, dihydroxide, bis(inner salt), hexasodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

96 hours

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

other: ASTM E 729-780 (1980) (Standard Practice for conducting acute toxicity tests with fishes, macroinvertebrate, and amphibians)

GLP compliance:

yes

Test organisms (species):

Cyprinus carpio

Details on test organisms:

- Common name: Common carp
- Source: Wu Sha Tow Fresh Water Fish Culture Demonstration Center, R.O.C.
- Age at study initiation: 60-90 days old
- Length at study initiation: 2-4 cm
- Weight at study initiation: 2.0 ± 0.1 g
- Acclimation period: at least seven days

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Test temperature:

25 °C

Details on test conditions:

- Test vessel: Glass tank, 23 cm (id) × 30 cm (h)
- Test volume: 10 L
- Aeration: Gently
- Renewal rate of test solution: 24 hours
- Photoperiod: 12-hours light

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Sublethal observations / clinical signs:

Table 1. Survival of common carp (Cyprinus carpio) exposed to EVERZOL BLUE ED-G for 96 hours in preliminary test

Nominal Concentration (mg/L)	No. of Survival Fish
	0 hr	3 hr	24 hr	48 hr	72 hr	96 hr
100	10	10	10	10	10	10
10	10	10	10	10	10	10
1.0	10	10	10	10	10	10
Control	10	10	10	10	10	10

Table 2. Survival of common carp (Cyprinus carpio) exposed to EVERZOL BLUE ED-G for 96 hoursin definitive test

Nominal Concentration (mg/L)	No. of Survival Fish*
	0 hr	3 hr	24 hr	48 hr	72 hr	96 hr
100	30	30	30	30	30	30
Control	30	30	30	30	30	30

*: There was no abnormal behavior observed.

 

Table 3. Water quality measurements during the acute toxicity test of EVERZOL BLUE ED-G to common carp (Cyprinus carpio) in definitive test

Nominal Concentration (mg/L)	Exposure Time
	3 hr	24 hr	48 hr	72 hr	96 hr
pH
100	8.4 ± 0.0	8.4 ± 0.0	8.3 ± 0.0	8.4 ± 0.1	8.4 ± 0.0
Control	8.4 ± 0.0	8.3 ± 0.0	8.3 ± 0.0	8.3 ± 0.0	8.3 ± 0.1
dissolved oxygen (mg/L)*
100	6.8 ± 0.0	7.0 ± 0.3	6.9 ± 0.3	7.7 ± 0.1	7.4 ± 0.2
Control	6.9 ± 0.2	6.6 ± 0.3	7.1 ± 0.8	7.5 ± 0.1	7.4 ± 0.1
conductivity (μscm^-1)
100	544 ± 10	561 ± 5	523 ± 14	524 ± 5	516 ± 25
Control	507 ± 8	529 ± 5	483 ± 7	489 ± 10	481 ± 17
mg/L calcium as CaCO3
100	57 ± 3	58 ± 5	57 ± 1	50 ± 2	58 ± 2
Control	59 ± 1	59 ± 1	58 ± 1	54 ± 1	56 ± 0

*: Air saturation value at 25°C: 8.26 mg/L

 

Table 4. The acute toxicity of EVERZOL BLUE ED-G to common carp (Cyprinus carpio)

Parameter	Exposure Time
	24 hr	48 hr	72 hr	96 hr
LC50	> 100	> 100	> 100	> 100

Conclusions:

According to ASTM E 729-780 and OECD 202 test method, the 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L.
No adverse effects were reported.

Executive summary:

This test using the procedures outlined in the TACTRI Study Plan for EF3G1090.7 (07AQC321) which is based on the ASTM E 729-780 (ASTM, 1980) and OECD 202 (OECD, 1981). The values of water quality measurements during definitive test were considered adequate for testing. Throughout the test period, the colors of all the test solutions were deep blue except controls treatment. There was no abnormal behavior observed in all exposure levels during testing. Under the conditions of this test, the 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L.

Description of key information

The 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L for common carp (ASTM E 729-780 and OECD TG202).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The values of water quality measurements contain pH (8.3-8.4), dissolved oxygen concentrations (6.6-7.7 mg/L), conductivity (481-561 μscm^-1) and total hardness (50-59 mg/L as CaCO3) during definitive test were considered adequate for testing. Throughout the test period, the colors of all the test solutions were deep blue except controls treatment. There was no abnormal behavior observed in all exposure levels during testing. Under the conditions of this test, the 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L.