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Biodegradation in water

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical Pyridinium, 1,1'-[(6,13-dichloro-4,11-disulfo-3,10-triphenodioxazinediyl)bis[imino-2,1-ethanediylimino[6-[(2,5-disulfophenyl)amino]-1,3,5-triazine-4,2-diyl]]]bis[3-carboxy-, dihydroxide, bis(inner salt), hexasodium salt can be expected to be range from 0 to 1.2% by using CO2 evolution & DOC removal parameter in 28 to 35 days, respectively and BOD5 can be expected to be 0 g O2/g test material. Thus, based on this, test chemical Pyridinium, 1,1'-[(6,13-dichloro-4,11 -disulfo-3,10 -triphenodioxazinediyl)bis[imino-2,1 -ethanediylimino[6 -[(2,5 -disulfophenyl)amino]-1,3,5 -triazine-4,2 -diyl]]]bis[3-carboxy-, dihydroxide, bis(inner salt), hexasodium salt is considered to be not readily biodegradable in nature.

Additional information

Biodegradation in water

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the biodegradability of the test chemical Pyridinium, 1,1'-[(6,13-dichloro-4,11- disulfo-3,10 -triphenodioxazinediyl)bis[imino-2,1-ethanediylimino[6-[(2,5-disulfophenyl)amino]-1,3,5-triazine-4,2-diyl]]]bis[3-carboxy-, dihydroxide, bis(inner salt), hexasodium salt (CAS no. 79771 -28 -1). The studies are as mentioned below:

 

Biodegradation study was carried out using river die away test for 35 days for evaluating the percentage biodegradability of test substance. Initial test substance conc. used was 1 mg/l (1000 ppb). The percentage degradation of test substance was determined to be 0% after 35 days by using CO2 evolution parameter. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

Biodegradation study was conducted for 5 days for evaluating the percentage biodegradability of test substance. Bacteria was used as a test inoculum for the study. The 5 day BOD value of test chemical was determined to be 0 g O2/g test material. Thus, based on this BOD5 value, test substance is considered to be not readily biodegradable in nature.

 

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance. The study was performed according to EPA OTS 796.3180 (Ready Biodegradability: Modified AFNOR Test) and OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test), respectively, under aerobic conditions. Domestic sewage effluentwas used as a test inoculum obtained from sewage treatment plant. Initial test substance concentration used for the study was 40 mg/l based on DOC (Dissolved Organic Carbon). Modifications of the guideline involve sterilisation instead of sterile filtration and bacterial concentration of 2.6000000 per ml, respectively.The percentage degradation of test substance was determined to be 1.2% by using DOC removal parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical Pyridinium, 1,1'-[(6,13-dichloro-4,11-disulfo-3,10-triphenodioxazinediyl)bis[imino-2,1-ethanediylimino[6-[(2,5-disulfophenyl)amino]-1,3,5-triazine-4,2-diyl]]]bis[3-carboxy-, dihydroxide, bis(inner salt), hexasodium salt can be expected to be range from 0 to 1.2% by using CO2 evolution & DOC removal parameter in 28 to 35 days, respectively and BOD5 can be expected to be 0 g O2/g test material. Thus, based on this, test chemical Pyridinium, 1,1'-[(6,13-dichloro-4,11-disulfo-3,10-triphenodioxazinediyl)bis[imino-2,1-ethanediylimino[6-[(2,5-disulfophenyl)amino]-1,3,5 -triazine-4,2 -diyl]]]bis[3 -carboxy-, dihydroxide, bis(inner salt), hexasodium salt is considered to be not readily biodegradable in nature.

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